Invitrogen

Collagen IV Monoclonal Antibody (1042), eBioscience™

Collagen IV Antibody in Immunohistochemistry (Paraffin) (IHC (P))

图: 1 / 7

Collagen IV Antibody (14-9871-82) in IHC (P)

Immunohistochemistry on formalin-fixed paraffin embedded human placenta using 5 µg/mL of Mouse IgG2b Isotype Control (left) or 5 µg/mL of Anti-Human Collagen Type IV Purified (right) followed by biotinylated Anti-Mouse IgG, and DAB visualization.Nuclei are counterstained with hematoxylin.

Collagen IV Antibody in Immunocytochemistry (ICC/IF)

Collagen IV Antibody in Immunohistochemistry (IHC)

Product Image Gallery

产品信息

14-9871-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-Dependent

免疫组化 (IHC)

查看3篇已发表文章 3篇已发表文章

免疫组化（石蜡） (IHC (P))

5 µg/mL

免疫组化（冰冻） (IHC (F))

5 µg/mL

免疫细胞化学 (ICC/IF)

查看4篇已发表文章 4篇已发表文章

免疫沉淀 (IP)

Assay-Dependent

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

1042

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10870985

产品详细信息

Description: This monoclonal antibody 1042 recognizes human collagen Type IV. Collagens are the main components of the extracellular matrix and provide the tensile strength to tissues. There are four main types of collagen (I, II, III and IV) which contribute to the connective tissue. Expression of type IV is restricted to the basal lamina. It contains two alpha-1 and one alpha-2-like chains. Collagen Type IV is associated with disorders such as Alport syndrome and Goodpasture's syndrome.

Applications Reported: This 1042 antibody has been reported for use in immunoprecipitation, western blotting, immunohistochemical staining of frozen (IHC-F) and formalin-fixed paraffin embedded (IHC-P) tissue sections.

Applications Tested: This 1042 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded (FFPE) tissue with low pH antigen retrieval. This can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Collagen IV is a type of collagen protein that is a major component of the basement membrane in various tissues, including the kidneys, lungs, and skin. It forms a network-like structure that provides support and stability to the surrounding tissue. Collagen IV is composed of three alpha chains, which are encoded by the COL4A1, COL4A2, and COL4A3 genes. Mutations in these genes can lead to various genetic disorders, such as Alport syndrome and Goodpasture syndrome, which affect the kidneys and lungs, respectively.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Transglutaminase 2: Development of therapeutic antibodies reveals four inhibitory epitopes and confirms extracellular function in fibrotic remodelling.

British journal of pharmacology

Maamra M,Benayad OM,Matthews D,Kettleborough C,Atkinson J,Cain K,Bon H,Brand H,Parkinson M,Watson PF,Johnson TS

14-9871-82 was used in Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry to develop a high affinity TG2-specific antibody against extracellular TG2 activity, with characteristics suitable for therapeutic development.

Wed Jun 01 00:00:00 EDT 2022

SeqStain is an efficient method for multiplexed, spatialomic profiling of human and murine tissues.

Cell reports methods

Rajagopalan A,Venkatesh I,Aslam R,Kirchenbuechler D,Khanna S,Cimbaluk D,Kordower JH,Gupta V

Published figure using Collagen IV monoclonal antibody (Product # 14-9871-82) in Immunocytochemistry

Mon Jun 21 00:00:00 EDT 2021

Effect of Fibrin Concentration on the In Vitro Production of Dermo-Epidermal Equivalents.

International journal of molecular sciences

Montero A,Quílez C,Valencia L,Girón P,Jorcano JL,Velasco D

14-9871-82 was used in Immunohistochemistry-immunofluorescence to determine the optimal final fibrin concentration for designing a functional skin dermo-epidermal equivalent for in vitro applications.

Wed Jun 23 00:00:00 EDT 2021

A Personalized Glomerulus Chip Engineered from Stem Cell-Derived Epithelium and Vascular Endothelium.

Micromachines

Roye Y,Bhattacharya R,Mou X,Zhou Y,Burt MA,Musah S

14-9871-82 was used in Immunocytochemistry to engineer a personalized glomerulus chip system reconstituted from human induced pluripotent stem (iPS) cell-derived vascular endothelial cells (ECs) and podocytes from a single patient.

Mon Aug 16 00:00:00 EDT 2021

Expression of metalloproteinases MMP-2 and MMP-9 is associated to the presence of androgen receptor in epithelial ovarian tumors.

Journal of ovarian research

Morales-Vásquez F,Castillo-Sánchez R,Gómora MJ,Almaraz MÁ,Pedernera E,Pérez-Montiel D,Rendón E,López-Basave HN,Román-Basaure E,Cuevas-Covarrubias S,Maldonado-Cubas J,Villa A,Mendez C

14-9871 was used in Immunohistochemistry to show that MMP-2 located in the epithelium and the stroma are independent prognostic biomarkers for overall survival in epithelial ovarian tumors.

Tue Jul 28 00:00:00 EDT 2020

Spontaneous Extracellular Matrix Accumulation in a Human in vitro Model of Renal Fibrosis Is Mediated by αV Integrins.

Nephron

Bon H,Hales P,Lumb S,Holdsworth G,Johnson T,Qureshi O,Twomey BM

Published figure using Collagen IV monoclonal antibody (Product # 14-9871-82) in Immunocytochemistry

Tue Sep 01 00:00:00 EDT 2020

Spontaneous Extracellular Matrix Accumulation in a Human in vitro Model of Renal Fibrosis Is Mediated by αV Integrins.

Nephron

Bon H,Hales P,Lumb S,Holdsworth G,Johnson T,Qureshi O,Twomey BM

Published figure using Collagen IV monoclonal antibody (Product # 14-9871-82) in Immunocytochemistry

Tue Sep 01 00:00:00 EDT 2020

A high content, phenotypic 'scar-in-a-jar' assay for rapid quantification of collagen fibrillogenesis using disease-derived pulmonary fibroblasts.

BMC biomedical engineering

Good RB,Eley JD,Gower E,Butt G,Blanchard AD,Fisher AJ,Nanthakumar CB

Published figure using Collagen IV monoclonal antibody (Product # 14-9871-82) in Immunocytochemistry

Sat Apr 16 00:00:00 EDT 2022

生物信息学

蛋白别名: arresten; COL4A1 NC1 domain; Collagen 4; Collagen alpha-1(IV) chain; Collagen alpha-2(IV) chain; Collagen alpha-3(IV) chain; Collagen alpha-4(IV) chain; Collagen alpha-5(IV) chain; Collagen alpha-6(IV) chain; collagen IV, alpha-1 polypeptide; collagen IV, alpha-3 polypeptide; Collagen IV, alpha-4 polypeptide; collagen IV, alpha-5 polypeptide; collagen IV, alpha-6 polypeptide; collagen of basement membrane, alpha-1 chain; collagen of basement membrane, alpha-4 chain; collagen of basement membrane, alpha-5 chain; collagen of basement membrane, alpha-6; collagen, type IV, alpha 1; collagen, type IV, alpha 3 (Goodpasture antigen); collagen, type IV, alpha 4; collagen, type IV, alpha 5; collagen, type IV, alpha 6; dA149D17.3; dA24A23.1; dJ889N15.4 (Collagen Alpha 6(IV)); Goodpasture antigen; tumstatin; Type IV Collagen

查看更多收起

基因别名: ASLN; ATS; BSVD; CA44; CA54; COL4A1; COL4A2; COL4A3; COL4A4; COL4A5; COL4A6; CXDELq22.3; DELXq22.3; DFNX6; ICH; POREN2; RATOR

查看更多收起

功能

extracellular matrix structural constituent protein binding extracellular matrix constituent conferring elasticity platelet-derived growth factor binding integrin binding structural molecule activity metalloendopeptidase inhibitor activity extracellular matrix structural protein extracellular matrix protein

参与通路

patterning of blood vessels brain development neuromuscular junction development extracellular matrix organization collagen catabolic process epithelial cell differentiation blood vessel morphogenesis retinal blood vessel morphogenesis renal tubule morphogenesis cellular response to amino acid stimulus basement membrane organization activation of cysteine-type endopeptidase activity involved in apoptotic process cell adhesion cell surface receptor signaling pathway sensory perception of sound blood circulation cell proliferation negative regulation of cell proliferation negative regulation of endopeptidase activity negative regulation of angiogenesis glomerular basement membrane development endothelial cell apoptotic process

Collagen IV Monoclonal Antibody (1042), eBioscience™

Collagen IV Antibody (14-9871-82) in IHC (P)

产品信息

14-9871-82

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫组化（冰冻） (IHC (F))

免疫细胞化学 (ICC/IF)

免疫沉淀 (IP)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

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