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Invitrogen
PCNA Monoclonal Antibody (PC10 (3F81)), eBioscience™
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FIGURE: 1 / 6
PCNA Antibody (14-9910-82) in WB
Western blot was performed using Anti-PCNA Monoclonal Antibody (PC10 (3F81)), eBioscience™(Product # 14-9910-82) and a 32kDa band corresponding to PCNA was observed across all tested cell lines. Nuclear enriched extracts (30 µg lysate) of HeLa (Lane 1), MCF7 (Lane 2), Jurkat (Lane 3), HEK-293 (Lane 4), NIH/3T3 (Lane 5), MDA-MB-231 (Lane 6), A549 (Lane 7), SH-SY5Y (Lane 8), PC-12 (Lane 9) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0342BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane ... View More
Product Details
14-9910-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Chordate
Host/Isotype
Mouse
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
PC10 (3F81)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The PC10 antibody recognizes the proliferating cell nuclear antigen (PCNA), a 36 kDa protein, also known as polymerase delta auxiliary protein. PC10 antibody reacts with human, mouse, and rat PCNA. The peak expression of PCNA occurs during the S-phase.
Applications Reported: The PC10 (a.k.a. 3F81) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistochemical staining.
Applications Tested: The PC10 (a.k.a. 3F81) antibody has been tested by flow cytometric analysis. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The PC10 antibody has also been tested by immunocytochemistry of methanol-fixed MDCK cells and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
PCNA (polymerase delta auxiliary protein) is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. PCNA binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumor tissue (i.e. relevant to prognosis). PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. PCNA is found in the nucleus and is a cofactor of DNA polymerase delta, and acts as a homotrimer and helps increase the processing of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of PCNA have been described on chromosome 4 and on the X chromosome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Cyclin; DNA polymerase delta auxiliary protein; HGCN8729; MGC8367; PCNA; Proliferating cell nuclear antigen
Gene Aliases: ATLD2; PCNA; Pcna/cyclin; PCNAR
UniProt ID: (Human) P12004, (Rat) P04961, (Mouse) P17918
Entrez Gene ID: (Human) 5111, (Rat) 25737, (Mouse) 18538
purine-specific mismatch base pair DNA N-glycosylase activity
chromatin binding
damaged DNA binding
protein binding
enzyme binding
estrogen receptor binding
DNA polymerase processivity factor activity
receptor tyrosine kinase binding
dinucleotide insertion or deletion binding
MutLalpha complex binding
histone acetyltransferase binding
identical protein binding
DNA polymerase binding
transcription factor binding
DNA binding
DNA polymerase processivity factor
G1/S transition of mitotic cell cycle
regulation of transcription involved in G1/S transition of mitotic cell cycle
telomere maintenance via recombination
telomere maintenance
DNA strand elongation involved in DNA replication
leading strand elongation
transcription-coupled nucleotide-excision repair
nucleotide-excision repair, DNA incision, 5'-to lesion
nucleotide-excision repair, DNA gap filling
mismatch repair
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
heart development
cell proliferation
protein sumoylation
translesion synthesis
epithelial cell differentiation
replication fork processing
positive regulation of deoxyribonuclease activity
nucleotide-excision repair, DNA incision
response to lipid
cellular response to UV
error-prone translesion synthesis
DNA damage response, detection of DNA damage
positive regulation of DNA repair
positive regulation of DNA replication
response to cadmium ion
error-free translesion synthesis
mitotic telomere maintenance via semi-conservative replication
negative regulation of transcription from RNA polymerase II promoter
regulation of DNA replication
base-excision repair, gap-filling
cellular response to xenobiotic stimulus
DNA replication
DNA repair
cellular response to DNA damage stimulus
regulation of catalytic activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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