Search Thermo Fisher Scientific
Invitrogen
Description: The eBio17G10.2 monoclonal antibody reacts with human immunoglobulin-like transcript 7 (ILT7), also known as CD85g. ILT7 is part of the leukocyte immunoglobulin-like receptor (LIR) gene family and associates with FcεR1gamma to form a functional complex which transduces ITAM-mediated signals that negatively modulates TLR-induced type I IFN production by human plasmacytoid dendritic cells. ILT7 is expressed exclusively on plasmacytoid dendritic cells and is absent in monocyte derived DCs. Treatment with IL-3 or CpG causes a decrease in expression.
Double staining of human PBMCs with eBio17G10.2 and anti-pDC marker BDCA2 yields virtually complete co-staining. Cross linking of ILT7 with eBio17G10.2 antibody causes reduced transcription of IFN-alpha1, IFN-alpha2, IFN-alpha4 and IFN-beta.
Applications Reported: This eBio17G10.2 (17G10.2) antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBio17G10.2 (17G10.2) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
ILT7 is a pDC-specific IgG-like transcript and is a member of the leukocyte immunoglobulin-like receptor gene family. This receptor, in complex with FcER1a, transduces ITAM-mediated signals which negatively modulate type I IFN production by pDC. ILT7 is thus a potential target to regulate Type 1 IFN production by pDC, which play a major role during viral infection, auto-immune diseases and anti-cancer immunity. Additionally, ILT7, along with CD123 and BDCA-2, can be used to identify or classify subsets of pDCalong with TLR expression and TLR mediated immune responses.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: CD85 antigen-like family member G; CD85g; ILT-7; Immunoglobulin-like transcript 7; leucocyte Ig-like receptor A4; Leukocyte immunoglobulin-like receptor subfamily A member 4; leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 4; leukocyte immunoglobulin-like receptor, subfamily A (without TM domain), member 4
基因别名: CD85g; ILT7; LILRA4
UniProt ID: (Human) P59901
Entrez Gene ID: (Human) 23547
分子生物学功能: immunoglobulin receptor superfamily