Invitrogen

IDO Monoclonal Antibody (eyedio), APC, eBioscience™

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IDO Antibody (17-9477-42) in Flow

Normal human peripheral blood cells were stimulated overnight with LPS (Product # 00-4976-93) then surface stained with Anti-Human CD11c eFluor® 450 (Product # 48-0116-42) followed by fixation and permeabilization with the Intracellular Fixation and Permeabilization Buffer Set (Product # 88-8824-00). The cells were then intracellularly stained with Mouse IgG1 K Isotype Control APC (Product # 17-4714-82) (left) or Anti-Human IDO APC (right). Viable cells in the monocyte and lymphocyte gate, as determined by Fixable Viability Dye eFluor™ 780... View More

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产品信息

17-9477-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.06 µg)/test

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产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

eyedio

偶联物

APC APC APC

激发/发射光谱

651/660 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2716946

产品详细信息

Description: This eyedio monoclonal antibody reacts with human indoleamine-2,3-dioxygenase (IDO, INDO, IDO1), an intracellular enzyme that catalyzes the degradation of tryptophan to kynurenines. IDO is expressed in a wide variety of tissues and cells, including macrophages, plasmacytoid dendritic cells, and several cell lines. IDO can be induced in many different cell types by IFN gamma or other inflammatory stimuli. Expression of IDO in antigen presenting cells and tumors is thought to mediate immune suppression through depletion of this essential amino acid and/or by the creation of tryptophan metabolites that causeapoptosis of T cells and induction of regulatory T cells.

Applications Reported: This eyedio antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This eyedio antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells using the Intracellular Fixation and Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This antibody can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

靶标信息

IDO1 is an intracellular heme-containing enzyme that catalyzes the oxidative cleavage of the indole ring of several important regulatory molecules like tryptophan, serotonin, and melatonin. By doing this, IDO1 initiates the production of biologically active metabolites, commonly referred to as kynurenines. IDO1 is widely expressed in a variety of human tissues as well as in macrophages and dendritic cells (DCs). In inflammation, interferons (IFNs) act on specific receptors to trigger IDO1 induction. The production of IFN-gamma and induction of IDO1 represent important antimicrobial mechanisms. Degradation and depletion of tryptophan by IDO1 inhibits the growth of viruses, bacteria and parasites. Furthermore, IDO1 plays a complex and crucial role in immunoregulation during infection, pregnancy, autoimmunity, transplantation, and neoplasia.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Aberrant tryptophan metabolism leads to unfavorable outcomes in lenalidomide-treated myeloma patients.

Hematological oncology

Asano A,Ri M,Masaki A,Maeda Y,Tachita T,Hirade K,Marumo Y,Nakashima T,Hagiwara S,Kinoshita S,Suzuki T,Narita T,Kusumoto S,Komatsu H,Inagaki H,Iida S

Published figure using IDO monoclonal antibody (Product # 17-9477-42) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2023

Laeverin/aminopeptidase Q induces indoleamine 2,3-dioxygenase-1 in human monocytes.

iScience

Suzuki T,Iizuka T,Kagami K,Matsumoto T,Yamazaki R,Daikoku T,Horie A,Ono M,Hattori A,Fujiwara H

17-9477-42 was used in Flow Cytometry to suggest that LVRN is one of the key molecules that mediate the interaction between EVT and monocytes/macrophages and creates an immunosuppressive environment at the maternal-fetal interface in the uterus.

Fri Sep 15 00:00:00 EDT 2023

Mitochondrial interaction of fibrosis-protective 5-methoxy tryptophan enhances collagen uptake by macrophages.

Free radical biology & medicine

Maassen S,Warner H,Ioannidis M,Jansma J,Markus H,El Aidy S,Chiara MD,Chiara JL,Maierhofer L,Weavers H,van den Bogaart G

Published figure using IDO monoclonal antibody (Product # 17-9477-42) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2022

IL-10^-/- Enhances DCs Immunity Against Chlamydia psittaci Infection via OX40L/NLRP3 and IDO/Treg Pathways.

Frontiers in immunology

Li Q,Li X,Quan H,Wang Y,Qu G,Shen Z,He C

Published figure using IDO monoclonal antibody (Product # 17-9477-42) in Flow Cytometry

Mon Sep 27 00:00:00 EDT 2021

IL-10^-/- Enhances DCs Immunity Against Chlamydia psittaci Infection via OX40L/NLRP3 and IDO/Treg Pathways.

Frontiers in immunology

Li Q,Li X,Quan H,Wang Y,Qu G,Shen Z,He C

Published figure using IDO monoclonal antibody (Product # 17-9477-42) in Flow Cytometry

Mon Sep 27 00:00:00 EDT 2021

Development of a human umbilical cord-derived mesenchymal stromal cell-based advanced therapy medicinal product to treat immune and/or inflammatory diseases.

Stem cell research & therapy

Mebarki M,Iglicki N,Marigny C,Abadie C,Nicolet C,Churlaud G,Maheux C,Boucher H,Monsel A,Menasché P,Larghero J,Faivre L,Cras A

Published figure using IDO monoclonal antibody (Product # 17-9477-42) in Flow Cytometry

Sat Nov 13 00:00:00 EST 2021

Continuing Effect of Cytokines and Toll-Like Receptor Agonists on Indoleamine-2,3-Dioxygenase-1 in Human Periodontal Ligament Stem/Stromal Cells.

Cells

Behm C,Blufstein A,Gahn J,Kubin B,Moritz A,Rausch-Fan X,Andrukhov O

Published figure using IDO monoclonal antibody (Product # 17-9477-42) in Flow Cytometry

Wed Dec 16 00:00:00 EST 2020

Immune Checkpoint Inhibition Overcomes ADCP-Induced Immunosuppression by Macrophages.

Cell

Su S,Zhao J,Xing Y,Zhang X,Liu J,Ouyang Q,Chen J,Su F,Liu Q,Song E

17-9477-42 was used in Flow Cytometry to unveil a deleterious role of antibody-dependent cellular cytotoxicity macrophages in cancer immunosuppression.

Thu Oct 04 00:00:00 EDT 2018

生物信息学

蛋白别名: EC 1.13.11.52; IDO-1; indolamine 2,3 dioxygenase; indole 2,3-dioxygenase; indoleamine; Indoleamine 2,3-dioxygenase 1; Indoleamine-2; Indoleamine-pyrrole 2,3-dioxygenase

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基因别名: IDO; IDO-1; IDO1; INDO

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UniProt ID: (Human) P14902

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Entrez Gene ID: (Human) 3620

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功能

tryptophan 2,3-dioxygenase activity electron carrier activity heme binding indoleamine 2,3-dioxygenase activity metal ion binding oxygenase

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