Invitrogen

CD11b Monoclonal Antibody (ICRF44), PE-Cyanine7, eBioscience™

图: 1 / 31

CD11b Antibody (25-0118-42) in Flow

Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control PE-Cyanine7 (Product # 25-4714-80) (open histogram) or Anti-Human CD11b PE-Cyanine7 (filled histogram). Cells in the monocyte gate were used for analysis.

Product Image Gallery

产品信息

25-0118-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (1 µg)/test

查看36篇已发表文章 36篇已发表文章

产品规格

种属反应

Baboon, Chimpanzee, Cynomolgus monkey, Human, Rhesus monkey

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

ICRF44

偶联物

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

激发/发射光谱

569/780 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_1582272

产品详细信息

Description: The ICRF44 monoclonal antibody reacts with human CD11b, a 165 kDa adhesion molecule. CD11b associated with integrin beta2 (CD18) is expressed on the surface of monocytes, granulocytes, activated lymphocytes and a subset of NK cells. CD11b is a receptor for intercellular adhesion molecule family members CD54, CD102 and CD50 as well as for iC3b. These adhesions are crucial in cell-cell and cell-matrix interactions.

Applications Reported: This ICRF44 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This ICRF44 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

ELMO1 Deficiency Reduces Neutrophil Chemotaxis in Murine Peritonitis.

International journal of molecular sciences

Yu S,Geng X,Liu H,Zhang Y,Cao X,Li B,Yan J

Published figure using CD11b monoclonal antibody (Product # 25-0118-42) in Flow Cytometry

Sun Apr 30 00:00:00 EDT 2023

Regulation of synaptic connectivity in schizophrenia spectrum by mutual neuron-microglia interaction.

Communications biology

Breitmeyer R,Vogel S,Heider J,Hartmann SM,Wüst R,Keller AL,Binner A,Fitzgerald JC,Fallgatter AJ,Volkmer H

Published figure using CD11b monoclonal antibody (Product # 25-0118-42) in Flow Cytometry

Sat Apr 29 00:00:00 EDT 2023

LAL deficiency induced myeloid-derived suppressor cells as targets and biomarkers for lung cancer.

Journal for immunotherapy of cancer

Zhao T,Liu S,Hanna NH,Jalal S,Ding X,Wan J,Yan C,Du H

Published figure using CD11b monoclonal antibody (Product # 25-0118-42) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2023

SARS-CoV-2 Delta (B.1.617.2) variant replicates and induces syncytia formation in human induced pluripotent stem cell-derived macrophages.

PeerJ

Thaweerattanasinp T,Wanitchang A,Saenboonrueng J,Srisutthisamphan K,Wanasen N,Sungsuwan S,Jongkaewwattana A,Chailangkarn T

Published figure using CD11b monoclonal antibody (Product # 25-0118-42) in Flow Cytometry

Thu Mar 09 00:00:00 EST 2023

Methylation of SPRED1: A New Target in Acute Myeloid Leukemia.

Frontiers in oncology

Su N,Wang Y,Lu X,Xu W,Wang H,Mo W,Pang H,Tang R,Li S,Yan X,Li Y,Zhang R

Published figure using CD11b monoclonal antibody (Product # 25-0118-42) in Flow Cytometry

Sat Apr 02 00:00:00 EDT 2022

Prognostic Lnc-S100B-2 Affects Cell Apoptosis and Microenvironment of Colorectal Cancer through MLLT10 Signaling.

Journal of oncology

Yi J,Peng F,Zhao J,Gong X

Published figure using CD11b monoclonal antibody (Product # 25-0118-42) in Flow Cytometry

Tue Feb 08 00:00:00 EST 2022

miR-204-containing exosomes ameliorate GVHD-associated dry eye disease.

Science advances

Zhou T,He C,Lai P,Yang Z,Liu Y,Xu H,Lin X,Ni B,Ju R,Yi W,Liang L,Pei D,Egwuagu CE,Liu X

Published figure using CD11b monoclonal antibody (Product # 25-0118-42) in Flow Cytometry

Fri Jan 14 00:00:00 EST 2022

The Transcription of ZIP9 Is Associated With the Macrophage Polarization and the Pathogenesis of Hepatocellular Carcinoma.

Frontiers in immunology

Gou Y,Yang D,Tian T,Zhu X,Zhang R,Ren J,Tu D,Luo Y,Miao Y,Zhao H,Wang Y,Wei B

Published figure using CD11b monoclonal antibody (Product # 25-0118-42) in Flow Cytometry

Fri Apr 08 00:00:00 EDT 2022

Regional and age-related diversity of human mature oligodendrocytes.

Glia

Yaqubi M,Luo JXX,Baig S,Cui QL,Petrecca K,Desu H,Larochelle C,Afanasiev E,Hall JA,Dudley R,Srour M,Haglund L,Ouellet J,Georgiopoulos M,Santaguida C,Sonnen JA,Healy LM,Stratton JA,Kennedy TE,Antel JP

25-0118-42 was used in Flow cytometry/Cell sorting to indicate heterogeneity of MOL populations in the human CNS that may reflect developmental and environmental influences.

Sat Oct 01 00:00:00 EDT 2022

Heterogeneous disease-propagating stem cells in juvenile myelomonocytic leukemia.

The Journal of experimental medicine

Louka E,Povinelli B,Rodriguez-Meira A,Buck G,Wen WX,Wang G,Sousos N,Ashley N,Hamblin A,Booth CAG,Roy A,Elliott N,Iskander D,de la Fuente J,Fordham N,O'Byrne S,Inglott S,Norfo R,Salio M,Thongjuea S,Rao A,Roberts I,Mead AJ

Published figure using CD11b monoclonal antibody (Product # 25-0118-42) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

IgE-Based Therapeutic Combination Enhances Antitumor Response in Preclinical Models of Pancreatic Cancer.

Molecular cancer therapeutics

Markov SD,Caffrey TC,O'Connell KA,Grunkemeyer JA,Shin S,Hanson R,Patil PP,Shukla SK,Gonzalez D,Crawford AJ,Vance KE,Huang Y,Eberle KC,Radhakrishnan P,Grandgenett PM,Singh PK,Madiyalakan R,Daniels-Wells TR,Penichet ML,Nicodemus CF,Poole JA,Jaffee EM,Hollingsworth MA,Mehla K

25-0118-42 was used in Flow cytometry/Cell sorting to demonstrate the novel tumor protective benefits of tumor antigen-specific IgE-based therapeutics in a preclinical model of pancreatic cancer, which can open new avenues for future clinical interventions.

Wed Dec 01 00:00:00 EST 2021

Different Induction of PD-L1 (CD274) and PD-1 (CD279) Expression in THP-1-Differentiated Types 1 and 2 Macrophages.

Journal of inflammation research

Lai CY,Tseng PC,Chen CL,Satria RD,Wang YT,Lin CF

Published figure using CD11b monoclonal antibody (Product # 25-0118-42) in Flow Cytometry

Wed Apr 27 00:00:00 EDT 2022

G-MDSCs promote aging-related cardiac fibrosis by activating myofibroblasts and preventing senescence.

Cell death & disease

Sun SN,Ni SH,Li Y,Liu X,Deng JP,Chen ZX,Li H,Feng WJ,Huang YS,Li DN,Xian SX,Yang ZQ,Wang LJ,Lu L

Published figure using CD11b monoclonal antibody (Product # 25-0118-42) in Flow Cytometry

Tue Jun 08 00:00:00 EDT 2021

G-MDSCs promote aging-related cardiac fibrosis by activating myofibroblasts and preventing senescence.

Cell death & disease

Sun SN,Ni SH,Li Y,Liu X,Deng JP,Chen ZX,Li H,Feng WJ,Huang YS,Li DN,Xian SX,Yang ZQ,Wang LJ,Lu L

Published figure using CD11b monoclonal antibody (Product # 25-0118-42) in Flow Cytometry

Tue Jun 08 00:00:00 EDT 2021

Intestinal CD11b⁺ B Cells Ameliorate Colitis by Secreting Immunoglobulin A.

Frontiers in immunology

Fu Y,Wang Z,Yu B,Lin Y,Huang E,Liu R,Zhao C,Lu M,Xu W,Liu H,Liu Y,Wang L,Chu Y

25-0118-42 was used in Flow Cytometry to demonstrate that CD11b+ B cells are essential intestinal suppressive immune cells and the primary source of intestinal IgA, which plays an indispensable role in maintaining intestinal homeostasis.

Fri Jan 07 00:00:00 EST 2022

Associations between Hypertriglyceridemia and Circulating Neutrophil Subpopulation in Patients with Dyslipidemia.

International journal of inflammation

Genkel V,Dolgushin I,Baturina I,Savochkina A,Kuznetsova A,Pykhova L,Shaposhnik I

Published figure using CD11b monoclonal antibody (Product # 25-0118-42) in Flow Cytometry

Sun Apr 24 00:00:00 EDT 2022

Dominant-negative NFKBIA mutation promotes IL-1β production causing hepatic disease with severe immunodeficiency.

The Journal of clinical investigation

Tan EE,Hopkins RA,Lim CK,Jamuar SS,Ong C,Thoon KC,Koh MJ,Shin EM,Lian DW,Weerasooriya M,Lee CZ,Soetedjo AAP,Lim CS,Au VB,Chua E,Lee HY,Jones LA,James SS,Kaliaperumal N,Kwok J,Tan ES,Thomas B,Wu LX,Ho L,Fairhurst AM,Ginhoux F,Teo AK,Zhang YL,Ong KH,Yu W,Venkatesh B,Tergaonkar V,Reversade B,Chin KC,Tan AM,Liew WK,Connolly JE

25-0118 was used in Flow cytometry/Cell sorting to reveal a previously unrecognized role of human IκBα as an essential regulator of canonical NF-κB signaling in the prevention of neutrophil-dependent autoinflammatory diseases.

Mon Nov 02 00:00:00 EST 2020

Single-Cell Analyses Reveal Megakaryocyte-Biased Hematopoiesis in Myelofibrosis and Identify Mutant Clone-Specific Targets.

Molecular cell

Psaila B,Wang G,Rodriguez-Meira A,Li R,Heuston EF,Murphy L,Yee D,Hitchcock IS,Sousos N,O'Sullivan J,Anderson S,Senis YA,Weinberg OK,Calicchio ML,Iskander D,Royston D,Milojkovic D,Roberts I,Bodine DM,Thongjuea S,Mead AJ

Published figure using CD11b monoclonal antibody (Product # 25-0118-42) in Flow Cytometry

Thu May 07 00:00:00 EDT 2020

The long non-coding RNA Cancer Susceptibility 15 (CASC15) is induced by isocitrate dehydrogenase (IDH) mutations and maintains an immature phenotype in adult acute myeloid leukemia.

Haematologica

Grasedieck S,Ruess C,Krowiorz K,Lux S,Pochert N,Schwarzer A,Klusmann JH,Jongen-Lavrencic M,Herold T,Bullinger L,Pollack JR,Rouhi A,Kuchenbauer F

Published figure using CD11b monoclonal antibody (Product # 25-0118-42) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2020

Galectin-9 promotes a suppressive microenvironment in human cancer by enhancing STING degradation.

Oncogenesis

Zhang CX,Huang DJ,Baloche V,Zhang L,Xu JX,Li BW,Zhao XR,He J,Mai HQ,Chen QY,Zhang XS,Busson P,Cui J,Li J

Published figure using CD11b monoclonal antibody (Product # 25-0118-42) in Flow Cytometry

Mon Jul 06 00:00:00 EDT 2020

Connecting METTL3 and intratumoural CD33⁺ MDSCs in predicting clinical outcome in cervical cancer.

Journal of translational medicine

Ni HH,Zhang L,Huang H,Dai SQ,Li J

Published figure using CD11b monoclonal antibody (Product # 25-0118-42) in Flow Cytometry

Thu Oct 15 00:00:00 EDT 2020

Ubiquitin-specific peptidase 3 induces TPA-mediated leukemia cell differentiation via regulating H2AK119ub.

Animal cells and systems

Chae YC,Jung H,Kim JY,Lee DH,Seo SB

Published figure using CD11b monoclonal antibody (Product # 25-0118-42) in Flow Cytometry

Tue Sep 29 00:00:00 EDT 2020

Microbiome Influences Prenatal and Adult Microglia in a Sex-Specific Manner.

Cell

Thion MS,Low D,Silvin A,Chen J,Grisel P,Schulte-Schrepping J,Blecher R,Ulas T,Squarzoni P,Hoeffel G,Coulpier F,Siopi E,David FS,Scholz C,Shihui F,Lum J,Amoyo AA,Larbi A,Poidinger M,Buttgereit A,Lledo PM,Greter M,Chan JKY,Amit I,Beyer M,Schultze JL,Schlitzer A,Pettersson S,Ginhoux F,Garel S

Published figure using CD11b monoclonal antibody (Product # 25-0118-42) in Flow Cytometry

Thu Jan 25 00:00:00 EST 2018

AURKA Suppresses Leukemic THP-1 Cell Differentiation through Inhibition of the KDM6B Pathway.

Molecules and cells

Park JW,Cho H,Oh H,Kim JY,Seo SB

Published figure using CD11b monoclonal antibody (Product # 25-0118-42) in Flow Cytometry

Thu May 31 00:00:00 EDT 2018

n-butanol extract from Folium isatidis inhibits the lipopolysaccharide-induced downregulation of CXCR1 and CXCR2 on human neutrophils.

Molecular medicine reports

Wu B,Wang L,Jiang L,Dong L,Xu F,Lu Y,Jin J,Wang Z,Liang G,Shan X

Published figure using CD11b monoclonal antibody (Product # 25-0118-42) in Flow Cytometry

Mon Jan 01 00:00:00 EST 2018

Accumulation of T-helper 22 cells, interleukin-22 and myeloid-derived suppressor cells promotes gastric cancer progression in elderly patients.

Oncology letters

Chen X,Wang Y,Wang J,Wen J,Jia X,Wang X,Zhang H

Published figure using CD11b monoclonal antibody (Product # 25-0118-42) in Flow Cytometry

Sun Jul 01 00:00:00 EDT 2018

Deacetylase activity-independent transcriptional activation by HDAC2 during TPA-induced HL-60 cell differentiation.

PloS one

Jung H,Kim JY,Kim KB,Chae YC,Hahn Y,Kim JW,Seo SB

Published figure using CD11b monoclonal antibody (Product # 25-0118-42) in Flow Cytometry

Thu Feb 07 00:00:00 EST 2019

Microglia innately develop within cerebral organoids.

Nature communications

Ormel PR,Vieira de Sá R,van Bodegraven EJ,Karst H,Harschnitz O,Sneeboer MAM,Johansen LE,van Dijk RE,Scheefhals N,Berdenis van Berlekom A,Ribes Martínez E,Kling S,MacGillavry HD,van den Berg LH,Kahn RS,Hol EM,de Witte LD,Pasterkamp RJ

Published figure using CD11b monoclonal antibody (Product # 25-0118-42) in Flow Cytometry

Tue Oct 09 00:00:00 EDT 2018

The prognostic significance of CD11b⁺CX3CR1⁺ monocytes in patients with newly diagnosed diffuse large B-cell lymphoma.

Oncotarget

Yhim HY,Kim JA,Ko SH,Park Y,Yim E,Kim HS,Kwak JY

Published figure using CD11b monoclonal antibody (Product # 25-0118-42) in Flow Cytometry

Fri Nov 03 00:00:00 EDT 2017

CD19 CAR immune pressure induces B-precursor acute lymphoblastic leukaemia lineage switch exposing inherent leukaemic plasticity.

Nature communications

Jacoby E,Nguyen SM,Fountaine TJ,Welp K,Gryder B,Qin H,Yang Y,Chien CD,Seif AE,Lei H,Song YK,Khan J,Lee DW,Mackall CL,Gardner RA,Jensen MC,Shern JF,Fry TJ

25-0118 was used in Flow cytometry/Cell sorting to study how CD19 CAR immune pressure induces a B-precursor acute lymphoblastic leukaemia lineage switch.

Wed Jul 27 00:00:00 EDT 2016

Metabolic regulation of hepatitis B immunopathology by myeloid-derived suppressor cells.

Nature medicine

Pallett LJ,Gill US,Quaglia A,Sinclair LV,Jover-Cobos M,Schurich A,Singh KP,Thomas N,Das A,Chen A,Fusai G,Bertoletti A,Cantrell DA,Kennedy PT,Davies NA,Haniffa M,Maini MK

Published figure using CD11b monoclonal antibody (Product # 25-0118-42) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2015

STK4 regulates TLR pathways and protects against chronic inflammation-related hepatocellular carcinoma.

The Journal of clinical investigation

Li W,Xiao J,Zhou X,Xu M,Hu C,Xu X,Lu Y,Liu C,Xue S,Nie L,Zhang H,Li Z,Zhang Y,Ji F,Hui L,Tao W,Wei B,Wang H

Published figure using CD11b monoclonal antibody (Product # 25-0118-42) in Flow Cytometry

Mon Nov 02 00:00:00 EST 2015

Induction of autophagy is a key component of all-trans-retinoic acid-induced differentiation in leukemia cells and a potential target for pharmacologic modulation.

Experimental hematology

Orfali N,O'Donovan TR,Nyhan MJ,Britschgi A,Tschan MP,Cahill MR,Mongan NP,Gudas LJ,McKenna SL

Published figure using CD11b monoclonal antibody (Product # 25-0118-42) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2015

dNP2 is a blood-brain barrier-permeable peptide enabling ctCTLA-4 protein delivery to ameliorate experimental autoimmune encephalomyelitis.

Nature communications

Lim S,Kim WJ,Kim YH,Lee S,Koo JH,Lee JA,Yoon H,Kim DH,Park HJ,Kim HM,Lee HG,Yun Kim J,Lee JU,Hun Shin J,Kyun Kim L,Doh J,Kim H,Lee SK,Bothwell ALM,Suh M,Choi JM

Published figure using CD11b monoclonal antibody (Product # 25-0118-42) in Flow Cytometry

Tue Sep 15 00:00:00 EDT 2015

Heparin-binding epidermal growth factor-like growth factor (HB-EGF) preserves gut barrier function by blocking neutrophil-endothelial cell adhesion after hemorrhagic shock and resuscitation in mice.

Surgery

Zhang HY,James I,Chen CL,Besner GE

Published figure using CD11b monoclonal antibody (Product # 25-0118-42) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2012

Normal human monocytes exposed to glioma cells acquire myeloid-derived suppressor cell-like properties.

Neuro-oncology

Rodrigues JC,Gonzalez GC,Zhang L,Ibrahim G,Kelly JJ,Gustafson MP,Lin Y,Dietz AB,Forsyth PA,Yong VW,Parney IF

Published figure using CD11b monoclonal antibody (Product # 25-0118-42) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2010

An adventitial IL-6/MCP1 amplification loop accelerates macrophage-mediated vascular inflammation leading to aortic dissection in mice.

The Journal of clinical investigation

Tieu BC,Lee C,Sun H,Lejeune W,Recinos A,Ju X,Spratt H,Guo DC,Milewicz D,Tilton RG,Brasier AR

25-0118 was used in Flow cytometry/Cell sorting to identify a vascular inflammatory pathway that contributes to the development of cardiovascular disease.

Tue Dec 01 00:00:00 EST 2009

生物信息学

蛋白别名: antigen CD11b (p170); CD11 antigen-like family member B; CD11b; Cell surface glycoprotein MAC-1 subunit alpha; complement component 3 receptor 3 subunit; CR-3 alpha chain; integrin alpha M; Integrin alpha-M; integrin, alpha M (complement component 3 receptor 3 subunit); Leukocyte adhesion receptor MO1; Mac-1 alpha (Mac1A); MAC-1 alpha subunit; macrophage antigen alpha polypeptide; MGC117044; Neutrophil adherence receptor; neutrophil adherence receptor alpha-M subunit; unnamed protein product

查看更多收起

基因别名: CD11B; CR3A; ITGAM; MAC-1; MAC1A; MO1A; SLEB6

查看更多收起

UniProt ID: (Human) P11215