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Invitrogen
CD101 Monoclonal Antibody (Moushi101), PE-Cyanine7, eBioscience™
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FIGURE: 1 / 3
CD101 Antibody (25-1011-82) in Flow
Staining of C57Bl/6 bone marrow cells with Anti-Mouse Ly-6G (Gr-1) FITC (Product # 11-5931-82) and 0.125 µg of Rat IgG2a K Isotype Control PE-Cyanine7 (Product # 25-4321-82) (left) or 0.125 µg of Anti-Mouse CD101 PE-Cyanine7 (right). Cells in the granulocyte gate were used for analysis.
Product Details
25-1011-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
C. elegans,
Human,
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
Moushi101
Conjugate
Excitation/Emission Max
569/780 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The monoclonal antibody moushi101 recognizes mouse CD101, also known as IGSF2 and V7 (7 IgV domains). CD101 is a transmembrane glycoprotein containing 7 Ig-like IgV domains and a short cytoplasmic tail with several phosphorylation sites. Cross-linking CD101 can antagonize TCR/CD3-mediated lymphocyte activation. CD101 is expressed on monocytes, granulocytes, dendritic cells, Langerhans cells and activated T cells. In addition, CD101 is found on a subset of CD25+Foxp3+ T regulatory cells that showed higher suppressive properties in vitro and in vivo.
Applications Reported: This Moushi101 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This Moushi101 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD101, also known as the V7 antigen, is a transmembrane glycoprotein and a member of the immunoglobulin superfamily. It is characterized by 7 Ig-like IgV domains and a short cytoplasmic tail with several phosphorylation sites. CD101 forms a disulfide-linked homodimer, although its precise function remains unknown. Expression of CD101 is observed on monocytes, granulocytes, dendritic cells, Langerhans cells, and activated T cells. It is also found on a subset of CD25+Foxp3+ regulatory T cells, which exhibit higher suppressive properties in vitro and in vivo. CD101 plays a role in modulating immune responses. Cross-linking CD101 can antagonize TCR/CD3-mediated lymphocyte activation, while CD101+ CD28+ T cells are highly responsive to CD28 signaling. In combination with anti-CD28 or suboptimal levels of anti-CD3, anti-CD101 can enhance proliferation, suggesting an activating role. The monoclonal antibody BB27 has been shown to inhibit T cell reactivity in allogeneic and antigen-specific mixed DC-T cell cultures. In mice, CD101 is expressed on a subpopulation of regulatory T cells (CD4+, CD25+, Foxp3+) with high suppressor activity. In humans, CD101 expression on peripheral blood mononuclear cells (PBMCs) stains approximately 30% of Foxp3+ cells, although studies have not confirmed higher suppressor activity in the human CD101+ population.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: CD101; EWI immunoglobulin subfamily member EWI-101; EWI-101; Glu-Trp-Ile EWI motif-containing protein 101; IgSF2; Immunoglobulin superfamily member 2; immunoglobulin superfamily, member 2; leukocyte surface protein; RP11-27K13.2
Gene Aliases: Cd101; EWI-101; Gm1016; Gm734; Igsf2
UniProt ID: (Mouse) A8E0Y8
Entrez Gene ID: (Mouse) 630146
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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