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Invitrogen
IRF8 Monoclonal Antibody (V3GYWCH), PE-Cyanine7, eBioscience™
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FIGURE: 1 / 3
IRF8 Antibody (25-9852-82) in Flow
Surface staining of normal human peripheral blood cells with Anti-Human CD123 Monoclonal Antibody, APC (Product # 17-1239-42), followed by intracellular staining using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol with Mouse IgG1 kappa Isotype Control, PE-Cyanine7 (Product # 25-4714-80) (left) or IRF8 Monoclonal Antibody, PE-Cyanine7 (right). Cells in the lymphocyte plus monocyte gate were used for analysis.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
25-9852-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
V3GYWCH
Conjugate
PE-Cyanine7
PE-Cyanine7
PE-Cyanine7
Excitation/Emission Max
569/780 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The V3GYWCH monoclonal antibody reacts with human and mouse interferon regulatory factor 8 (IRF8; ICSBP). IRF8 is a 50 kDa transcription factor that plays a critical role in the development of dendritic cells (DC). Specifically, IRF8 is essential in the development and differentiation of plasmacytoid DC (pDC) and CD8a+ DC. IRF8-/- mice are deficient in both pDC and CD8a+ DC populations. CD8a- DC are present in normal numbers in IRF8-/- mice but fail to mature upon Toll-like receptor signaling and are functionally impaired in response to in vivo microbial stimulation. IRF8 deficiency does not affect the frequency or viability of DC precursors and as a result retroviral IRF8 transduction restores pDC development from DC progenitors in IRF8-/- mice.
Applications Reported: This V3GYWCH antibody has been reported for use in flow cytometric analysis.
Applications Tested: This V3GYWCH antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Target Information
IRF8 (interferon regulatory factor 8) is a transcriptional activator or repressor. It specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes. IRF8 plays a negative regulatory role in cells of the immune system. It is involved in CD8+ dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells. This leads to recognition of AICE sequence, an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of the genes. Mutations in the gene can result in immunodeficiencey 32A and 32B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: H-ICSBP; ICSBP; Interferon consensus sequence-binding protein; Interferon regulatory factor 8; IRF-8
Gene Aliases: AI893568; ICSBP; ICSBP1; IRF-8; IRF8; Myls
UniProt ID: (Mouse) P23611
Entrez Gene ID: (Mouse) 15900
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