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Invitrogen
CD303a Monoclonal Antibody (201A), Brilliant Ultra Violet™ 395, eBioscience™
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CD303a Antibody (363-9818-42) in Flow
Normal human peripheral blood cells were stained with CD123 Monoclonal Antibody, APC (Product # 17-1239-42) and Mouse IgG2a kappa Isotype Control, Brilliant Ultra Violet 395 (Product # 363-4724-81) (left) or CD303a Monoclonal Antibody, Brilliant Ultra Violet 395 (right). Viable cells in the monocyte and lymphocyte gate were used for analysis, as determined by 7-AAD (Product # 00-6993-50).
产品信息
363-9818-42
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
201A
偶联物
Brilliant Ultra Violet™ 395
Brilliant Ultra Violet™ 395
Brilliant Ultra Violet™ 395
激发/发射光谱
347/399 nm
查看光谱
形式
liquid
浓度
5 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with BSA
内含物
0.09% sodium azide
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Description
This 201A monoclonal antibody reacts with human CD303a, which is also known as CLEC4C or Blood Dendritic Cell Antigen 2 (BDCA-2). This type II transmembrane protein is expressed almost exclusively on plasmacytoid dendritic cells, which secrete type I interferon during antiviral and antitumor responses. Studies have demonstrated that CD303a binds asialo-galactosyl-oligosaccharides on invading pathogens, resulting in the activation of signaling pathways that lead to CD303a internalization and downregulation of interferon production.
Applications Tested
This 201A antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Blocking Buffers
When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) or Brilliant Stain Buffer (Product # 00-4409-75) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information.
Fixation
• Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation.
• Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 350 nm; Emission: 395 nm; Laser: Ultraviolet Laser.
BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.
靶标信息
This gene encodes a member of the C-type lectin/C-type lectin-like domain superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type 2 transmembrane protein may play a role in dendritic cell function. Two transcript variants encoding distinct isoforms have been identified for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: BDCA-2; Blood dendritic cell antigen 2; blood dendritic cell antigen 2 protein; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 11; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 7; C-type lectin; C-type lectin domain family 4 member C; C-type lectin receptor; DLEC; C-type lectin receptor; DLEC-b; C-type lectin superfamily member 7; CD303; CD303 antigen; dendritic cell lectin b; Dendritic lectin; unnamed protein product
基因别名: BDCA-2; BDCA2; CD303; CLEC4C; CLECSF11; CLECSF7; DLEC; HECL; PRO34150; UNQ9361/PRO34150
UniProt ID: (Human) Q8WTT0
Entrez Gene ID: (Human) 170482
Disclaimer
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