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Invitrogen
VSIG4 Monoclonal Antibody (JAV4), Brilliant Violet™ 605, eBioscience™
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FIGURE: 1 / 1
VSIG4 Antibody (406-5757-42) in Flow
Human monocyte-derived macrophages were stained with CD11b Monoclonal Antibody, FITC (Product # 11-0118-42) and Mouse IgG2a kappa Isotype Control, Brilliant Violet 605 (Product # 406-4724-81) (left) or VSIG4 Monoclonal Antibody, Brilliant Violet 605 (right). Viable cells in the monocyte gate were used for analysis, as determined by 7-AAD (Product # 00-6993-50).
Product Details
406-5757-42
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
JAV4
Immunogen
Fc-Protein and CHO cells
Conjugate
Excitation/Emission Max
406/603 nm
View spectra
Form
liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description
This JAV4 monoclonal antibody recognizes human V-Set and Immunoglobulin domain containing 4 (VSIG4), also known as Complement Receptor of the Immunoglobulin superfamily (CRIg) or Z39Ig. There are two variants of human VSIG4 that result from alternative splicing. Isoform 1 is longer and more prevalent than isoform 2, and this JAV4 antibody recognizes both. VSIG4 is a type I transmembrane glycoprotein structurally related to the B7 family of immune regulatory proteins. It contains one complete V-type Ig domain and one truncated C-type Ig domain. The JAV4 antibody will recognize VSIG4 on cells that have been formaldehyde-fixed and permeabilized. This antibody does not cross-react with mouse or rat VSIG4.
Applications Tested
This JAV4 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Blocking Buffers
When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) or Brilliant Stain Buffer (Product # 00-4409-75) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information.
Light sensitivity
This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation
• Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation.
• Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
• Our internal testing suggests that Brilliant Violet™ 605 (BV605) is not compatible with methanol-based fixation.
Excitation: 407 nm; Emission: 605 nm; Laser: Violet Laser.
BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.
Target Information
T cell activation by APCs is positively and negatively regulated by members of the B7 family. VSIG4 is a strong negative regulator of murine and human T cell proliferation and IL-2 production.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Protein Aliases: complement receptor of the immunoglobulin superfamily; Ig superfamily protein; Protein Z39Ig; V-set and immunoglobulin domain-containing protein 4
Gene Aliases: CRIg; UNQ317/PRO362; VSIG4; Z39IG
UniProt ID: (Human) Q9Y279
Entrez Gene ID: (Human) 11326
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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