Invitrogen

CD200 Monoclonal Antibody (OX104), Brilliant Violet™ 711, eBioscience™

Name: CD200 Monoclonal Antibody (OX104), Brilliant Violet\u2122 711, eBioscience\u2122
Brand: Invitrogen
SKU: 407-9200-42

查看其他53个CD200抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)

图: 1 / 1

CD200 Antibody (407-9200-42) in Flow

Normal human peripheral blood cells were stained with CD19 Monoclonal Antibody, PE (Product # 12-0199-42) and Mouse IgG1 kappa Isotype Control, Brilliant Violet 711 (Product # 407-4714-81) (left) or CD200 Monoclonal Antibody, Brilliant Violet 711 (right). Viable cells in the lymphocyte gate were used for analysis, as determined by Fixable Viability Dye eFluor 780 (Product # 65-0865-18).

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产品信息

407-9200-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.5 µg)/test

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

OX104

偶联物

Brilliant Violet™ 711 Brilliant Violet™ 711 Brilliant Violet™ 711

激发/发射光谱

406/711 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with BSA

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_3666092

产品详细信息

Description
The monoclonal antibody OX104 recognizes human CD200 also known as OX2.

Applications Tested
This OX104 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Blocking Buffers
When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) or Brilliant Stain Buffer (Product # 00-4409-75) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information.

Light sensitivity
This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation
• Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation.
• Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
• Our internal testing suggests that Brilliant Violet™ 711 (BV711) is not compatible with methanol-based fixation.

Excitation: 407 nm; Emission: 713 nm; Laser: Violet Laser.

BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.

靶标信息

CD200 is a type-1 membrane glycoprotein in the immunoglobulin superfamily, featuring two immunoglobulin domains. It is widely expressed on various cells, including B and T cells, keratinocytes, nerve cells, follicular dendritic cells, ovarian cells, and trophoblasts. CD200 interacts with CD200R on myeloid cells, inhibiting macrophage activation, reducing IL-6 production, and attenuating lymphocyte activation. It also prevents mast cell degranulation and decreases TNF-alpha and IL-13 secretion, playing a role in immune regulation and maternal tolerance. Homologues of CD200 are found in herpesviruses and poxviruses, indicating a conserved immune modulation function. CD200 has multiple alternatively spliced transcript variants, contributing to its functional diversity. Diseases linked to CD200 dysfunction include Mantle Cell Lymphoma and Primary Hypertrophic Osteoarthropathy, highlighting its importance in health and disease.

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