Invitrogen

CD64 Monoclonal Antibody (X54-5/7.1), PerCP-eFluor™ 710, eBioscience™

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

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5 篇参考文献

查看其他70个CD64抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

图: 1 / 3

CD64 Antibody (46-0641-82) in Flow

BALB/c mouse bone marrow cells were Fc blocked with CD16/CD32 Antibody (Product # 14-0161-82) and normal mouse serum, and stained with CD11b Monoclonal Antibody, FITC (Product # 11-0112-82) and 0.25 µg of Mouse IgG1 kappa Isotype Control, PerCP-eFluor 710 (Product # 46-4714-82) (left) or 0.25 µg of CD64 Monoclonal Antibody, PerCP-eFluor 710 (right). All cells were used for analysis.

Product Image Gallery

CD64 Antibody — Staining of mouse bone marrow. As expected based on the known relative expression patterns, CD64 clone X54-5/7.1 stains monocytes with the highest intensity (purple histogram) and B cell precursors with lower intensity (orange and blue histograms). Details: Mouse bone marrow was Fc blocked, stained with CD64 (clone X54-5/7.1), and co-stained with CD45R (B220) (clone RA3-6B2) and CD43 (clone eBioR2/60). Cells in the indicated color gates were used for analysis. {RE}

产品信息

46-0641-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.5 µg/test

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

X54-5/7.1

抗原

BALB/c mouse CD64-human IgG Fc fusion protein

偶联物

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

激发/发射光谱

482/708 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2735016

产品详细信息

Description: This X54-5/7.1 monoclonal antibody recognizes allelic variants a and b of mouse CD64, a high affinity Fc binding receptor also known as Fc-gammaR1 or Fcgr1. Alloform a is present in the following mouse strains: C57BL/6, BALB/c, DBA/2, and alloform b in strains: C3H/HeJ, CBA/J, NZW, SJL/J, 129/SvJ. In addition this antibody has been reported positive in strains: AKR, ALR, BUB, C58, CE, HRS,MRL, MON, NZB, NZO, NZW, PL, SJL, ST and SWR. This clone X54-5/7.1 will not recognize the allelic variant d, present in NOD/Lt mice. This antibody has been reported to stain RAW264.7 cell line.

Applications Reported: This X54-5/7.1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This X54-5/7.1 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

靶标信息

CD64 (FCGR1A) encodes a protein that plays an important role in the immune response. CD64 is a high-affinity Fc-gamma receptor and is one of three related gene family members located on chromosome 1. CD64 plays an important role in the immune response and its dysfunction has been studied in cervical adenitis and tetrasomy 21.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Neonatal imprinting of alveolar macrophages via neutrophil-derived 12-HETE.

Nature

Pernet E,Sun S,Sarden N,Gona S,Nguyen A,Khan N,Mawhinney M,Tran KA,Chronopoulos J,Amberkar D,Sadeghi M,Grant A,Wali S,Prevel R,Ding J,Martin JG,Thanabalasuriar A,Yipp BG,Barreiro LB,Divangahi M

46-0641-82 was used in Flow cytometry/Cell sorting to highlight the complexity of prenatal RTM programming and reveal their dependency on in trans eicosanoid production by neutrophils for lifelong self-renewal.

Wed Feb 01 00:00:00 EST 2023

Circulating hemopexin modulates anthracycline cardiac toxicity in patients and in mice.

Science advances

Liu J,Lane S,Lall R,Russo M,Farrell L,Debreli Coskun M,Curtin C,Araujo-Gutierrez R,Scherrer-Crosbie M,Trachtenberg BH,Kim J,Tolosano E,Ghigo A,Gerszten RE,Asnani A

Published figure using CD64 monoclonal antibody (Product # 46-0641-82) in Flow Cytometry

Fri Dec 23 00:00:00 EST 2022

Tetraspanin CD82 restrains phagocyte migration but supports macrophage activation.

iScience

McGowan ENS,Wong O,Jones E,Nguyen J,Wee J,Demaria MC,Deliyanti D,Johnson CJ,Hickey MJ,McConville MJ,Wilkinson-Berka JL,Wright MD,Binger KJ

46-0641-82 was used in Flow cytometry/Cell sorting to reveal an important role for CD82 in restraining phagocyte infiltration and mediating their differentiation in response to stimulatory cues.

Fri Jul 15 00:00:00 EDT 2022

The Cytokine TGF-β Induces Interleukin-31 Expression from Dermal Dendritic Cells to Activate Sensory Neurons and Stimulate Wound Itching.

Immunity

Xu J,Zanvit P,Hu L,Tseng PY,Liu N,Wang F,Liu O,Zhang D,Jin W,Guo N,Han Y,Yin J,Cain A,Hoon MA,Wang S,Chen W

46-0641 was used in Flow cytometry/Cell sorting to investigate the mechanisms underlying itching associated with cutaneous wound healing, focusing on the contribution of soluble factors released during healing.

Tue Aug 18 00:00:00 EDT 2020

Airway Microbiota-Host Interactions Regulate Secretory Leukocyte Protease Inhibitor Levels and Influence Allergic Airway Inflammation.

Cell reports

Jaeger N,McDonough RT,Rosen AL,Hernandez-Leyva A,Wilson NG,Lint MA,Russler-Germain EV,Chai JN,Bacharier LB,Hsieh CS,Kau AL

46-0641 was used in Flow cytometry/Cell sorting to investigate how chronic colonization impacts mucosal immunity and the development of allergic airway inflammation (AAI).

Tue Nov 03 00:00:00 EST 2020

生物信息学

蛋白别名: CD64; CD64 antigen; Fc-gamma receptor 1; Fc-gamma RI; FcgRI; FcRI; FLJ18345; High affinity immunoglobulin gamma Fc receptor I; IgG Fc receptor I; IgG high affinity Fc receptor; RP11-196G18.2

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基因别名: AI323638; AV092959; CD64; Fcg1; FcgammaRI; Fcgr1; IGGHAFC

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UniProt ID: (Mouse) P26151

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Entrez Gene ID: (Mouse) 14129

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功能

IgG receptor activity IgG binding leukotriene receptor binding immunoglobulin receptor superfamily

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