Invitrogen

CD15 Monoclonal Antibody (MMA), eFluor™ 450, eBioscience™

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CD15 Antibody (48-0158-42) in Flow

Staining of normal human peripheral blood cells with Mouse IgM Isotype Control eFluor® 450 (blue histogram) or Anti-Human CD15 eFluor® 450 (purple histogram). Cells in the granulocyte gate were used for analysis.

CD15 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

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产品信息

48-0158-42

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

5 µL (0.125 µg)/test

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgM

分类

Monoclonal

类型

Antibody

克隆号

MMA

偶联物

eFluor™ 450 eFluor™ 450 eFluor™ 450

激发/发射光谱

405/445 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_1907348

产品详细信息

Description: The MMA monoclonal antibody reacts with human CD15, Lewis X. This 3-fucosyl-N-acetyllactosamine carbohydrate moiety is expressed by granulocytes. Monocytes express this structure at varying degree while lymphocytes are negative.

Applications Reported: This MMA antibody has been reported for use in flow cytometric analysis.

Applications Tested: This MMA antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin's disease.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

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Neutrophil extracellular traps induce tumor metastasis through dual effects on cancer and endothelial cells.

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Jiang ZZ,Peng ZP,Liu XC,Guo HF,Zhou MM,Jiang D,Ning WR,Huang YF,Zheng L,Wu Y

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Rahmani NE,Ramachandra N,Sahu S,Gitego N,Lopez A,Pradhan K,Bhagat TD,Gordon-Mitchell S,Pena BR,Kazemi M,Rao K,Giricz O,Maqbool SB,Olea R,Zhao Y,Zhang J,Dolatshad H,Tittrea V,Tatwavedi D,Singh S,Lee J,Sun T,Steidl U,Shastri A,Inoue D,Abdel-Wahab O,Pellagatti A,Gavathiotis E,Boultwood J,Verma A

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Murlentamab, a Low Fucosylated Anti-Müllerian Hormone Type II Receptor (AMHRII) Antibody, Exhibits Anti-Tumor Activity through Tumor-Associated Macrophage Reprogrammation and T Cell Activation.

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Prat M,Salon M,Allain T,Dubreuil O,Noël G,Preisser L,Jean B,Cassard L,Lemée F,Tabah-Fish I,Pipy B,Jeannin P,Prost JF,Barret JM,Coste A

48-0158 was used in Flow cytometry/Cell sorting to demonstrate here that the murlentamab opsonization of AMHRII-expressing ovarian tumor cells, in the presence of unstimulated- or tumor-associated macrophage (TAM)-like macrophages, significantly promotes macrophage-mediated ADCC and shifts the whole microenvironment towards a pro-inflammatory and anti-tumoral status, thus triggering anti-tumor activity.

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Loss of testosterone impairs anti-tumor neutrophil function.

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Small-Molecule Targeting of Oncogenic FTO Demethylase in Acute Myeloid Leukemia.

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Huang Y,Su R,Sheng Y,Dong L,Dong Z,Xu H,Ni T,Zhang ZS,Zhang T,Li C,Han L,Zhu Z,Lian F,Wei J,Deng Q,Wang Y,Wunderlich M,Gao Z,Pan G,Zhong D,Zhou H,Zhang N,Gan J,Jiang H,Mulloy JC,Qian Z,Chen J,Yang CG

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生物信息学

蛋白别名: 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase; alpha (1,3) fucosyltransferase, myeloid-specific; Alpha-(1,3)-fucosyltransferase 4; ELAM ligand fucosyltransferase; ELAM-1 ligand fucosyltransferase; Fuc-TIV; Fucosyltransferase 4; fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific); Fucosyltransferase IV; fucT-IV; FUTIV; Galactoside 3-L-fucosyltransferase; Lewis X; SSEA-1; stage-specific embryonic antigen 1

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基因别名: CD15; ELFT; FCT3A; FUC-TIV; FUT4; FUTIV; LeX; SSEA-1

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UniProt ID: (Human) P22083

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Entrez Gene ID: (Human) 2526

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功能

fucosyltransferase activity alpha-(1->3)-fucosyltransferase activity glycosyltransferase

参与通路

carbohydrate metabolic process protein glycosylation protein N-linked glycosylation fucosylation L-fucose catabolic process