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          • Primary Antibodies ›
          • AKT1 Antibodies

          Invitrogen

          Phospho-AKT1 (Ser473) Monoclonal Antibody (SDRNR), eFluor™ 450, eBioscience™

          Advanced Verification
          9 Published Figures
          10 References
          View all (142) AKT1 antibodies

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          Cite Phospho-AKT1 (Ser473) Monoclonal Antibody (SDRNR), eFluor™ 450, eBioscience™

          • Antibody Testing Data (1)
          • Published Figures (9)
          • Advanced Verification (1)
          Phospho-AKT1 (Ser473) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          Phospho-AKT1 (Ser473) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 11

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          Phospho-AKT1 (Ser473) Antibody (48-9715-42) in Flow

          Normal human peripheral blood cells were unstimulated (orange histogram) or were stimulated with Anti-Human CD3 and CD28 Functional Grade Purifieds (Product # 16-0037-81 and Product # 16-0289-81) (purple histogram). The cells were then intracellularly stained with Anti-Human/Mouse phospho-AKT (S473) eFluor® 450 and Anti-Human CD3 PerCP-Cyanine5-5 (Product # 45-0036-42) (left) or Anti-Human CD19 PE (Product ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          Phospho-AKT1 (Ser473) Antibody in Flow Cytometry (Flow)
          Phospho-AKT1 (Ser473) Antibody in Flow Cytometry (Flow)
          Phospho-AKT1 (Ser473) Antibody in Flow Cytometry (Flow)
          Phospho-AKT1 (Ser473) Antibody in Flow Cytometry (Flow)
          Phospho-AKT1 (Ser473) Antibody in Flow Cytometry (Flow)
          Phospho-AKT1 (Ser473) Antibody in Flow Cytometry (Flow)
          Phospho-AKT1 (Ser473) Antibody in Flow Cytometry (Flow)
          Phospho-AKT1 (Ser473) Antibody in Flow Cytometry (Flow)
          Phospho-AKT1 (Ser473) Antibody in Flow Cytometry (Flow)
          Phospho-AKT1 (Ser473) Antibody in Flow Cytometry (Flow)
          Phospho-AKT1 (Ser473) Antibody

          Product Details

          48-9715-42

          Applications
          Tested Dilution
          Publications

          Flow Cytometry (Flow)

          5 µL (0.5 µg)/test
          View 10 publications 10 publications
          Product Specifications

          Species Reactivity

          Human, Mouse

          Published species

          Human, Mouse

          Host/Isotype

          Mouse / IgG2a, kappa

          Recommended Isotype Control

          Mouse IgG2a kappa Isotype Control (eBM2a), eFluor™ 450, eBioscience™

          Class

          Monoclonal

          Type

          Antibody

          Clone

          SDRNR

          Conjugate

          eFluor™ 450 eFluor™ 450 eFluor™ 450
          • APC 
          • Brilliant UV 395
          • Brilliant Violet 711
          • FITC
          • PE 
          • PE-Cyanine7
          • Request custom conjugation

          Excitation/Emission Max

          405/445 nm View spectra spectra

          Form

          Liquid

          Concentration

          5 µL/Test

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2, with BSA

          Contains

          0.09% sodium azide

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2574125

          Product Specific Information

          Description: This SDRNR monoclonal antibody recognizes human and mouse AKT (also known as Protein Kinase B (PKB)) when phosphorylated on S473. AKT is a serine/threonine protein kinase that plays a key role in multiple cellular processes including metabolism, proliferation, apoptosis/survival, and migration. There are three homologous isoforms of AKT: AKT1, AKT2, and AKT3. AKT is activated by binding of its pleckstrin homology (PH) domain to membrane phospholipids and by phosphorylation. Phosphorylation of AKT at T308 by PDK1 and at S473 is required for full activation of this kinase. AKT promotes cell survival by inhibiting apoptosis via phosphorylation and inactivation of several targets including Bad, Foxo1, c-Raf, and caspase-9. Deregulation of AKT has been implicated as a major contributing factor in many types of cancer. AKT is negatively regulated by the phosphatase PTEN as well as by the chemical inhibitor LY294002. Specificity of this SDRNR clone was determined by ELISA, flow cytometry, and western blotting.

          Applications Reported:This SDRNR antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

          Applications Tested: This SDRNR antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood mononuclear cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

          eFluor™ 450 is an alternative to Pacific Blue™. eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochrome.

          Staining Protocol: All protocols work well for this monoclonal antibody. Use of Protocol A: Two-step protocol: intracellular (cytoplasmic) proteins allows for the greatest flexibility for detection of surface and intracellular (cytoplasmic) proteins. Use of Protocol B: One-step protocol: intracellular (nuclear) proteins is recommended for staining of transcription factors in conjunction with surface and phosphorylated intracellular (cytoplasmic) proteins. Protocol C: Two-step protocol: Fixation/Methanol allows for the greatest discrimination of phospho-specific signaling between unstimulated and stimulated samples, but with limitations on the ability to stain specific surface proteins (refer to "Clone Performance Following Fixation/Permeabilization" located in the BestProtocols Section under the Resources tab online). All Protocols can be found in the Flow Cytometry Protocols: "Staining Intracellular Antigens for Flow Cytometry Protocol" located in the BestProtocols® Section under the Resources tab online.

          Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

          Filtration: 0.2 µm post-manufacturing filtered.

          Target Information

          AKT1 (PKB alpha) is a serine/threonine kinase that regulates cell survival. The activated enzyme inhibits apoptosis and stimulates cell cycle progression by phosphorylating numerous targets in various cell types, including cancer cells. This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. The AKT pathway is a major target for cancer drug discovery.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          Bioinformatics

          Protein Aliases: AKT1 kinase; AKT1m; Akt1m protein; C-AKT; CAKT; PKB; PKB alpha; PKB beta; PKB gamma; PKBG; Protein kinase B; Protein kinase B alpha; protein kinase B-alpha; Proto-oncogene c-Akt; rac protein kinase alpha; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; RAC-PK-beta; RAC-PK-gamma; related to A and C kinases; serine-threonine protein kinase; Thymoma viral proto-oncogene; v-akt murine thymoma viral oncogene homolog 1; v-akt murine thymoma viral oncogene-like protein 1

          View more View less

          Gene Aliases: AKT; AKT1; CWS6; PKB; PKB-ALPHA; PKB/Akt; PKBalpha; PRKBA; RAC; RAC-ALPHA

          View more View less

          UniProt ID: (Human) P31749, (Mouse) P31750

          View more View less

          Entrez Gene ID: (Human) 207, (Mouse) 11651

          View more View less

          Function(s)
          protein kinase activity protein serine/threonine kinase activity protein serine/threonine/tyrosine kinase activity protein kinase C binding protein binding ATP binding phosphatidylinositol-3,4,5-trisphosphate binding kinase activity enzyme binding nitric-oxide synthase regulator activity GTPase activating protein binding identical protein binding phosphatidylinositol-3,4-bisphosphate binding protein phosphatase 2A binding 14-3-3 protein binding nucleotide binding transferase activity transferase activity, transferring phosphorus-containing groups protein kinase binding non-receptor serine/threonine protein kinase
          Process(es)
          protein import into nucleus, translocation osteoblast differentiation maternal placenta development positive regulation of protein phosphorylation positive regulation of endothelial cell proliferation glycogen biosynthetic process regulation of glycogen biosynthetic process glucose metabolic process translation regulation of translation cellular protein modification process protein phosphorylation negative regulation of protein kinase activity nitric oxide biosynthetic process activation-induced cell death of T cells inflammatory response cellular response to DNA damage stimulus response to oxidative stress signal transduction G-protein coupled receptor signaling pathway germ cell development aging cell proliferation insulin receptor signaling pathway apoptotic mitochondrial changes response to heat negative regulation of autophagy negative regulation of gene expression negative regulation of plasma membrane long-chain fatty acid transport positive regulation of fibroblast migration positive regulation of sodium ion transport positive regulation of glucose metabolic process negative regulation of endopeptidase activity regulation of neuron projection development regulation of phosphatidylinositol 3-kinase signaling glucose transport phosphorylation protein ubiquitination peptidyl-serine phosphorylation peptidyl-threonine phosphorylation spinal cord development cell projection organization cell differentiation protein catabolic process platelet activation hyaluronan metabolic process positive regulation of cell growth regulation of cell migration endocrine pancreas development T cell costimulation regulation of myelination positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle lipopolysaccharide-mediated signaling pathway negative regulation of fatty acid beta-oxidation positive regulation of endodeoxyribonuclease activity response to food positive regulation of cellular protein metabolic process peripheral nervous system myelin maintenance positive regulation of proteasomal ubiquitin-dependent protein catabolic process cellular response to insulin stimulus positive regulation of peptidyl-serine phosphorylation response to fluid shear stress intracellular signal transduction cellular response to vascular endothelial growth factor stimulus ERBB2 signaling pathway glucose homeostasis positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process regulation of mRNA stability protein kinase B signaling positive regulation of blood vessel endothelial cell migration positive regulation of nitric oxide biosynthetic process positive regulation of fat cell differentiation positive regulation of glycogen biosynthetic process positive regulation of epidermal growth factor receptor signaling pathway negative regulation of cell size negative regulation of proteolysis positive regulation of vasoconstriction positive regulation of transcription from RNA polymerase II promoter positive regulation of glucose import negative regulation of JNK cascade protein autophosphorylation positive regulation of lipid biosynthetic process insulin-like growth factor receptor signaling pathway phosphatidylinositol-mediated signaling regulation of nitric-oxide synthase activity positive regulation of nitric-oxide synthase activity positive regulation of sequence-specific DNA binding transcription factor activity striated muscle cell differentiation response to growth hormone mammary gland epithelial cell differentiation glycogen cell differentiation involved in embryonic placenta development labyrinthine layer blood vessel development response to UV-A cellular response to mechanical stimulus cellular response to epidermal growth factor stimulus cellular response to prostaglandin E stimulus cellular response to organic cyclic compound cellular response to hypoxia establishment of protein localization to mitochondrion maintenance of protein location in mitochondrion positive regulation of establishment of protein localization to plasma membrane negative regulation of release of cytochrome c from mitochondria cellular response to granulocyte macrophage colony-stimulating factor stimulus execution phase of apoptosis chemical synaptic transmission, postsynaptic negative regulation of protein kinase activity by protein phosphorylation positive regulation of protein localization to nucleus negative regulation of neuron death regulation of signal transduction by p53 class mediator regulation of cell cycle checkpoint negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway cellular response to nerve growth factor stimulus response to insulin-like growth factor stimulus negative regulation of extrinsic apoptotic signaling pathway in absence of ligand carbohydrate metabolic process glycogen metabolic process transport apoptotic process cytoskeleton organization multicellular organism development nervous system development carbohydrate transport response to hormone regulation of protein localization cellular response to decreased oxygen levels anagen cellular response to growth factor stimulus cellular response to peptide
          It has to be done as per old AB suggested Products section.
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