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Rockland
5-mC Monoclonal Antibody (33D3)
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5-mC Antibody (200-301-V02) in ICC/IF
Immunofluorescence Microscopy results of Mouse anti-5-mC antibody. Tissue: HeLa cells. Fixation: 4% formaldehyde in PBS for 10 min at RT. Retrieval: 0.5% Triton X-100 for 1 hr, treated with 2N HCl for 1 hr. Block: PBS/0.1% Triton X-100/1% BSA. Primary antibody: 5-mC antibody at 1:500 for 1 hr at RT (left). Secondary antibody: Goat anti-Mouse Alexa594 secondary antibody at 1:10,000 for 45 min at RT. Staining: 5-mC antibody as red fluorescent signal (left), DAPI blue (middle), merge of the two stainings (right).
产品信息
200-301-V02
应用
建议稀释比
已发表文章
产品规格
种属反应
Chemical
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
33D3
抗原
Anti-5-mC Antibody was produced in mice by repeated immunization with 5-methylcytosine.
偶联物
形式
Liquid
浓度
1.24 mg/mL
纯化类型
Size-exclusion - Gel filtration
保存液
PBS, pH 7.2
内含物
0.05% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
靶标信息
Modified base 5-methyl cytidine (5-MeCyd) is found in DNA of plants and vertebrates. DNA methylation is a DNA modification process, which is involved in the control of gene expression. Reports suggest that in tumours, DNA is frequently globally hypomethylated compared to the DNA from normal tissue.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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