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Invitrogen
Mouse IgM Monoclonal Antibody (II/41), eFluor™ 660, eBioscience™
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产品信息
50-5790-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
Fish,
Human,
Mouse
宿主/亚型
Rat
/ IgG2a, kappa
分类
Monoclonal
类型
Antibody
克隆号
II/41
偶联物
激发/发射光谱
651/668 nm
查看光谱
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: The II/41 monoclonal antibody reacts with the mu heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated II/41 can be used as a detection secondary for mouse IgM.
Applications Reported: This II/41 antibody has been reported for use in flow cytometric analysis, microscopy, immunohistochemical staining, and immunocytochemistry.
Applications Tested: This II/41 antibody has been tested by immunocytochemistry on fixed cells and by immunohistochemistry on formalin-fixed paraffin embedded sections that were stained first with a primary antibody of the mouse IgM isotype. For immunocytochemistry and immunohistochemistry, this can be used at less than or equal to 10 µg/mL. This II/41 antibody has also been tested by flow cytometric analysis of mouse bone marrow cells and can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody by carefully titrated for optimal performance in the assay of interest.
eFluor™ 660 is a replacement for Alexa Fluor™ 647. eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Mouse IgM is an immunoglobulin M antibody that is produced by B cells in response to an antigen. It is the first antibody produced during an immune response and is the largest immunoglobulin in the serum. Mouse IgM is composed of five heavy chains and five light chains, and it is involved in complement activation and agglutination of antigens. It plays a crucial role in the innate immune response and is important for neutralizing pathogens before the adaptive immune response is fully activated. Mouse IgM is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
基因别名: AI326478; Igh-6; Igh-M; Igh6; Igm; muH
Entrez Gene ID: (Mouse) 16019
