Search Thermo Fisher Scientific
Invitrogen
图: 1 / 3
Description: The 1RELAH5 monoclonal antibody reacts with mouse c-Rel. c-Rel is a 65 kDa hematopoietic lineage-restricted member of the NF-kB family of transcription factors. In the thymus, c-Rel is upregulated in regulatory T cells (Treg) when compared to other CD4+ thymocytes. c-Rel is believed to play a critical role in thymic Treg (nTreg) development, but is not necessary for TGF beta-mediated T reg (iTreg) differentiation. C-Rel is also involved in proliferation and survival in mature T cells.
Applications Reported: This 1RELAH5 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This 1RELAH5 antibody has been tested by intracellular staining and flow cytometric analysis of mouse thymocytes using the Foxp3 Fixation/Permeabilization Buffers (Product # 00-5521-00) and protocol. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
The REL gene encodes c-Rel, a transcription factor that is a member of the Rel/NFKB family, which also includes RELA., RELB (604758), NFKB1, and NFKB2. These proteins are related through a highly conserved N-terminal region termed the 'Rel domain, ' which is responsible for DNA binding, dimerization, nuclear localization, and binding to the NFKB inhibitor (Belguise and Sonenshein, 2007).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: BCD541; C-Rel protein; C-Rel proto-oncogene protein; gemin 1; gemin-1; proto-oncogene c-Rel; SMA; SMA1; SMA2; SMA3; SMA4; SMN; SMN2; SMNT
基因别名: c-Rel
Entrez Gene ID: (Mouse) 19696
分子生物学功能:
Rel homology transcription factor