Invitrogen

IL-13 Monoclonal Antibody (eBio13A), eFluor™ 660, eBioscience™

Name: IL-13 Monoclonal Antibody (eBio13A), eFluor\u2122 660, eBioscience\u2122
Brand: Invitrogen
SKU: 50-7133-82

高级验证

42个已发表图片

46 篇参考文献

查看其他65个IL-13抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

图: 1 / 44

IL-13 Antibody (50-7133-82) in Flow

BALB/c splenocytes were polarized under Th2 conditions for 3 days and then restimulated with Cell Stimulation Cocktail (plus protein transport inhibitors) for 5 hours. Following restimulation, cells were fixed and permeabilized and then intracellularly stained with Anti-Mouse CD4 FITC (Product # 11-0042-82) and 0.125 µg of Rat IgG1 K Isotype Control eFluor® 660 (Product # 50-4301-82) (left) or 0.125 µg of Anti-Mouse IL-13 eFluor® 660 (right). Total viable cells, as determined by Fixable Viability Dye eFluor® 450 (Product # 65-0863-14), wer... View More

IL-13 Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)

IL-13 Antibody in Flow Cytometry, ELISA (Flow, ELISA)

Product Image Gallery

IL-13 Antibody — Fig. 4 GITR engagement drives ILC2-derived secretion and M2 macrophage polarization. A cohort of Rag2 -/- mice were fed on HFD and either treated with DTA-1 (1 mg/mouse) or isotype control by intraperitoneal injection every four days, n = 8. a The frequency of CD45 + cells of the VAT stromal vascular fraction (SVF) was quantified by flow cytometry. b Representative flow cytometry plots of VAT Lin - CD45 + IL-7R + ST2 + ILC2s in each group after 14 weeks of treatment, and corresponding quantitation presented as the number of ILC2s per gram of VAT. c Representative flow cytometry plots of intracellular IL-5 (top panel) and IL-13 (bottom panel) in VAT ILC2s and corresponding quantitation after 14 weeks of treatment, presented as frequency of positive ILC2s. d Gating strategy of macrophages in mouse VAT as CD45 + CD11b hi F4/80 hi . e Quantification of the number of CD45 + CD11b hi F4/80 hi macrophages per gram of VAT. f Representative flow cytometry plots of CD45 + CD11b hi F4/80 hi CD206 + CD11c + M2 macrophages in VAT and corresponding quantitation after 14 weeks of treatment. Error bars are the mean +- SEM. Student's t -test, * p < 0.05, *** p < 0.001 {TM}

产品信息

50-7133-82

应用

建议稀释比

已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

0.25 µg/test

查看43篇已发表文章 43篇已发表文章

酶联免疫吸附实验 (ELISA)

查看1篇已发表文章 1篇已发表文章

中和实验 (Neu)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Mouse

已发表种属

C. elegans, Human, Mouse

宿主/亚型

Rat / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

eBio13A

偶联物

eFluor™ 660 eFluor™ 660 eFluor™ 660

激发/发射光谱

651/668 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2574279

产品详细信息

Description: The eBio13A antibody reacts with mouse IL-13. IL-13 is a cytokine produced mainly by Th2 cells, but also by antigen-primed CD8 T cells. IL-13 has a strong involvement in allergic inflammation and parasitic clearing and in cancer models has been shown to have either inhibitory or stimulatory activity depending on the tumor. In humans, IL-13 is found to play a role in isotype switching in B cells. IL-13 is implicating in down modulating macrophage activity, through the reduction of pro-inflammatory cytokines (IL-1, IL-6, IL-8, IL-10, IL-12)

Applications Reported: This eBio13A antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This eBio13A antibody has been tested by intracellular staining and flow cytometric analysis of Th2 polarized mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and is involved in regulating inflammatory and immune responses. IL-13 down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines, and is involved in allergen-induced asthma through mechanisms independent of IgE and eosinophils. IL-13 also regulates several stages of B cell maturation and differentiation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. Structurally, IL-13 closely resembles IL-3, IL-4, IL-5 and GM-CSF. The gene encoding IL-13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with the IL-13 gene particularly close to IL4.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

生物信息学

蛋白别名: ALRH; ALRH antibody; BHR1; il 13; IL-13; ILN; Interleukin; Interleukin-13; Interleukin13; MGC116786; MGC116788; MGC116789; NC30 antibody; P600 antibody; T-cell activation protein P600

查看更多收起

基因别名: Il-13; Il13

查看更多收起

UniProt ID: (Mouse) P20109

查看更多收起

Entrez Gene ID: (Mouse) 16163

查看更多收起

功能

cytokine activity cytokine receptor binding interleukin-13 receptor binding

参与通路