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Invitrogen
Myosin 4 Monoclonal Antibody (MF20), Alexa Fluor™ 488, eBioscience™
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Myosin 4 Antibody (53-6503-82) in ICC/IF
Immunofluorescence analysis of MYH4 was performed using 70% confluent log phase C2C12 and C2C12 differentiated cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Myosin 4 Monoclonal Antibody (MF20), Alexa Fluor 488, eBioscience™ (Product # 53-6503-82) at 1:100 in 0.1% BSA, incubated at 4 degree Celsius overnight (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Dia... View More
产品信息
53-6503-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Bovine,
Dog,
Chicken,
Chimpanzee,
Cat,
Guinea pig,
Human,
Mouse,
Non-human primate,
Rabbit,
Rat
已发表种属
C. elegans,
Human,
Mouse
宿主/亚型
Mouse
/ IgG2b, kappa
分类
Monoclonal
类型
Antibody
克隆号
MF20
偶联物
激发/发射光谱
499/520 nm
查看光谱
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: This MF20 monoclonal antibody recognizes the heavy chain of myosin II, specificially the light meromyosin portion, in cardiac and skeletal muscle of vertebrates. Myosin II is composed of two heavy chains and four light chains. The 220-kDa myosin heavy chain exists as four different isoforms due to alternative splicing. Myosins interact with actin and hydrolyze ATP to function in muscle contraction, cytokinesis, and phagocytosis.
The MF20 has been shown to react to myosin from a variety of mammalian, avian and amphibian species, including rat, mouse, human, chicken, zebrafish, and dog.
Applications Reported: This MF20 antibody has been reported for use in immunohistochemical staining, immunocytochemistry, and immunohistochemical staining of frozen tissue sections.
Applications Tested: This MF20 antibody has been tested by immunocytochemistry on fixed and permeabilized C2C12 cells that were differentiated for 2 days in low serum medium prior to staining. Staining can be performed using less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Target protein includes a fast myosin isoform expressed in skeletal muscle and has function involved in Muscle contraction.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 2B myosin heavy chain; fast myosin heavy chain 2B; myh4; MyHC IIb isoform; MyHC-2b; MyHC-IIb; Myosin heavy chain 2b; myosin heavy chain 2X; Myosin heavy chain 4; Myosin heavy chain IIb; myosin heavy chain type IIb; Myosin heavy chain, skeletal muscle; Myosin heavy chain, skeletal muscle, fetal; Myosin heavy chain, skeletal muscle, juvenile; myosin, heavy chain 4, skeletal muscle; myosin, heavy polypeptide 4, skeletal muscle; Myosin-4; unnamed protein product
基因别名: AI506973; MHC; MHC2B; Minimsc; Minmus; MM; MYH-2B; MYH1; MYH2B; MYH4; MyHC-2B; MyHC-IIb; Myhsf
UniProt ID: (Human) Q9Y623, (Dog) Q076A5, (Rabbit) Q28641, (Mouse) Q5SX39, (Rat) Q29RW1
