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          • Primary Antibodies ›
          • SOX9 Antibodies

          Invitrogen

          SOX9 Monoclonal Antibody (GMPR9), Alexa Fluor™ 488, eBioscience™

          View all (49) SOX9 antibodies

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          Cite SOX9 Monoclonal Antibody (GMPR9), Alexa Fluor™ 488, eBioscience™

          Additional Information:
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          • Antibody Testing Data (1)
          SOX9 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          SOX9 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          SOX9 Antibody (53-9765-80) in ICC/IF

          Immunocytochemistry of fixed and permeabilized HepG2 cells using 10 µg/mL of Mouse IgG1 K Isotype Control Alexa Fluor® 488 (green, left) or 10 µg/mL of Anti-Human/Mouse Sox9 Alexa Fluor® 488 (green, right). Nuclei are stained with DAPI (blue), colocalization of signal appears aqua. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          View Product

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          SOX9 Antibody in Immunocytochemistry (ICC/IF)

          Product Details

          53-9765-80

          Applications
          Tested Dilution
          Publications

          Immunohistochemistry (Paraffin) (IHC (P))

          Assay-Dependent
          -

          Immunocytochemistry (ICC/IF)

          10 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse

          Host/Isotype

          Mouse / IgG1, kappa

          Recommended Isotype Control

          Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 488, eBioscience™

          Class

          Monoclonal

          Type

          Antibody

          Clone

          GMPR9

          Conjugate

          Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488
          • Unconjugated
          • Request custom conjugation

          Excitation/Emission Max

          499/520 nm View spectra spectra

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2

          Contains

          0.09% sodium azide

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2574462

          Product Specific Information

          Description: This GMPR9 monoclonal antibody reacts with human and mouse Sox9, a member of the Sry HMG-box (Sox) gene family of transcription factors. Like Sry, Sox9 contains a high mobility group (HMG-box) DNA-binding domain. The Sox9 DNA-binding domain recognizes the sequence AGAACAATGG, and when bound to co-factors, functions to bend the DNA. This structural change is thought to be important for chromatin remodeling and the initiation of more broad transcriptional regulation of multiple genes. Sox9 is expressed in multiple tissue types during development and its activation of downstream targets is determined by interaction with tissue-specific co-factors. During development, Sox9 expression is important for cartilage formation (mesenchymal to chondrocyte), sex determination (testes formation), neural crest formation and retinal development. Sox9 plays a role in the differentiation of multiple cell types including glial, hair, cardiac, melanocytes, pancreatic, prostate, and intestinal epithelium. Sox9 regulates expression of the following family member genes sox5, sox6, and sox10, in addition to collagen type 2 alpha, collagen 9A1, CEACAM1, and snail2. Sox9 dysregulation is involved in craniofacial defects, cleft palate, sex reversal, and some types of cancer (melanoma, lung adenocarcinoma, and breast cancer). Sox9 has also been shown to be expressed in adult stem cells in the liver, pancreas and intestine.

          Applications Reported: This GMPR9 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry.

          Applications Tested: This GMPR9 antibody has been tested by immunocytochemistry of fixed and permeabilized cells and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

          Filtration: 0.2 µm post-manufacturing filtered.

          Target Information

          SOX9 has a role in sex determination and differentiation of Sertoli cells. It is involved in chondrogenesis and regulates the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes. Translocation of this gene can cause campomelic dysplasia. SOX9 is involved in the formation of testes from the indifferent fetal gonads. It is a major molecular component of the neuron-glia switch in developing spinal cord. During normal development, SOX9 allows the prostate epithelium to outgrow into the mesenchyme and then provides basal cell support for development and maintenance of the luminal epithelium. These functions of SOX9 are subverted in prostate cancer to support tumor growth and invasion. SOX9 may direct the formation of neural crest precursors and the development of a range of neural crest derivative. It has a transcriptional regulation in melanin production in cells. Northern blot analysis shows its expression on adult testis, adult heart, and fetal brain.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: mSox9; SOX 9; SOX9; SRY (sex determining region Y)-box9; SRY (sex-determining region Y)-box 9 protein; SRY-box 9; SRY-Box 9 ; SRY-box containing gene 9; SRY-related HMG-box, gene 9; Transcription factor SOX-9; unnamed protein product

          View more View less

          Gene Aliases: 2010306G03Rik; AV220920; CMD1; CMPD1; ENH13; mKIAA4243; Sox-9; SOX9; SRA1; SRXX2; SRXY10; TES; TESCO

          View more View less

          UniProt ID: (Human) P48436, (Mouse) Q04887

          View more View less

          Entrez Gene ID: (Human) 6662, (Mouse) 20682

          View more View less

          Function(s)
          transcription regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding beta-catenin binding protein kinase A catalytic subunit binding bHLH transcription factor binding sequence-specific DNA binding pre-mRNA intronic binding sequence-specific double-stranded DNA binding core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding enhancer sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding protein kinase activity enhancer binding transcription regulatory region DNA binding protein heterodimerization activity
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter skeletal system development cartilage condensation cell fate specification epithelial to mesenchymal transition tissue homeostasis positive regulation of protein phosphorylation hair follicle development positive regulation of mesenchymal cell proliferation chondrocyte development negative regulation of immune system process heart valve development heart valve morphogenesis heart valve formation endocardial cushion morphogenesis chondrocyte differentiation involved in endochondral bone morphogenesis chondrocyte hypertrophy nucleosome assembly chromatin remodeling transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter protein complex assembly protein phosphorylation cytoskeleton organization signal transduction epidermal growth factor receptor signaling pathway Notch signaling pathway spermatogenesis heart development positive regulation of cell proliferation negative regulation of cell proliferation male gonad development regulation of cell cycle process positive regulation of gene expression negative regulation of gene expression positive regulation of epithelial cell migration neural crest cell development positive regulation of phosphatidylinositol 3-kinase signaling single organismal cell-cell adhesion male germ-line sex determination cAMP-mediated signaling cell differentiation regulation of cell adhesion extracellular matrix organization male sex determination negative regulation of ossification negative regulation of bone mineralization negative regulation of epithelial cell differentiation positive regulation of epithelial cell differentiation otic vesicle formation endocrine pancreas development negative regulation of chondrocyte differentiation positive regulation of chondrocyte differentiation lacrimal gland development protein localization to nucleus somatic stem cell population maintenance intrahepatic bile duct development regulation of cell proliferation negative regulation of apoptotic process protein kinase B signaling cell fate commitment regulation of cell differentiation negative regulation of myoblast differentiation positive regulation of protein catabolic process negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of photoreceptor cell differentiation oligodendrocyte differentiation homeostasis of number of cells within a tissue positive regulation of epithelial cell proliferation negative regulation of epithelial cell proliferation cartilage development Sertoli cell differentiation Sertoli cell development astrocyte fate commitment retina development in camera-type eye retinal rod cell differentiation endochondral bone morphogenesis epithelial tube branching involved in lung morphogenesis lung epithelial cell differentiation prostate gland morphogenesis epithelial cell proliferation involved in prostatic bud elongation bronchus cartilage development trachea cartilage development intestinal epithelial structure maintenance regulation of cell proliferation involved in tissue homeostasis positive regulation of cartilage development regulation of branching involved in lung morphogenesis morphogenesis of a branching epithelium lung smooth muscle development negative regulation of biomineral tissue development ERK1 and ERK2 cascade Harderian gland development cellular response to mechanical stimulus cellular response to retinoic acid cellular response to epidermal growth factor stimulus cellular response to heparin cellular response to transforming growth factor beta stimulus renal vesicle induction ureter development ureter smooth muscle cell differentiation ureter morphogenesis metanephric nephron tubule formation negative regulation of canonical Wnt signaling pathway cochlea morphogenesis positive regulation of kidney development positive regulation of branching involved in ureteric bud morphogenesis positive regulation of extracellular matrix assembly positive regulation of male gonad development positive regulation of cell proliferation involved in heart morphogenesis positive regulation of mesenchymal stem cell differentiation regulation of epithelial cell proliferation involved in lung morphogenesis negative regulation of mesenchymal cell apoptotic process ossification branching involved in ureteric bud morphogenesis morphogenesis of an epithelium chondrocyte differentiation glandular epithelial cell differentiation aortic valve morphogenesis growth plate cartilage chondrocyte growth apoptotic process central nervous system development cell proliferation mesenchymal cell proliferation gene expression regulation of gene expression neural crest cell fate specification glial cell fate specification bone mineralization prostate gland development mammary gland development notochord development regulation of apoptotic process negative regulation of osteoblast differentiation negative regulation of fatty acid oxidation neuron fate specification epithelial cell proliferation canonical Wnt signaling pathway limb bud formation Type I pneumocyte differentiation intestinal epithelial cell differentiation cell proliferation involved in heart morphogenesis protein-containing complex assembly response to fatty acid cellular response to interleukin-1 otic vesicle development cellular response to BMP stimulus stem cell proliferation metanephric tubule development ureter urothelium development extracellular matrix assembly anterior head development mesenchymal cell apoptotic process cell-cell adhesion positive regulation of chondrocyte proliferation negative regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of beta-catenin-TCF complex assembly positive regulation of stem cell proliferation
          It has to be done as per old AB suggested Products section.

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