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Identifyn
53BP1 Polyclonal Antibody, Alexa Fluor™ 488, Identifyn™
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FIGURE: 1 / 8
53BP1 Antibody (DC-000063) in ICC/IF
Identifyn Detailed protocols can be found below under Documents & Downloads. Airyscan: HeLa cells were grown on acid-etched 1.5H cover glass to ~50% confluency, then fixed in -20°C methanol and blocked with Thermo Fisher Fish Serum Blocker (Product # 37527X3). The primary antibody, Rabbit anti-Human 53BP1 Polyclonal - Identifyn SRM Alexa Fluor 488, IgG, was incubated for 1 hour at room temperature at a 1:250 dilution. Cells were washed 3 times in PBS. The second primary antibody, Nuclear Pore Complex Monoclonal Antibody (39C7), was incubat... View More
Product Details
DC-000063
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptide Sequences between Residues 360 - 410 and 1940 - 1970, referencing Uniprot Q12888 and Gene ID 7158.
Conjugate
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
50 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 10% glycerol
Contains
0.02% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Identifyn™ Primary and Secondary antibodies are extensively validated in Immunofluorescence assays by traditional as well as high resolution acquisition like Widefield SIM, Confocal, Airyscan and STORM Microscopy.
Recommended dilution for traditional microscopy:
Widefield - 1:250-1:1,000
Widefield SIM - 1:250-1:1,000
Confocal - 1:250-1:1,000
Recommended dilution for Super Resolution microscopy:
SIM (Structured Illumination Microscopy) 1:100-1:1,000
Single Molecule STORM (Stochastic Optical Reconstruction Microscopy) - 1:100-1:1,000
Detailed protocols can be found below under Documents & Downloads.
Target Information
53BP1 (P53-binding protein 1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase. Upon DNA damage, 53BP1 is phosphorylated and localizes to the presumptive sites of damage, specifically, double-strand breaks. 53BP1 may have a role in checkpoint signaling during mitosis, enhances TP53-mediated transcriptional activation, and participates in DNA repair by maintaining genomic stability.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 53BP1; FLJ41424; MGC138366; p53-binding protein 1; TP53-binding protein 1; tumor protein 53-binding protein, 1; tumor suppressor p53-binding protein 1; unnamed protein product
Gene Aliases: 53BP1; p202; p53BP1; TDRD30; TP53BP1
UniProt ID: (Human) Q12888
Entrez Gene ID: (Human) 7158
p53 binding
DNA binding
damaged DNA binding
transcription cofactor activity
transcription coactivator activity
protein binding
telomeric DNA binding
histone binding
sequence-specific DNA binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
ubiquitinated histone binding
histone H4K20me2 reader activity
histone reader activity
histone H4K20me methyltransferase activity
DNA damage checkpoint
DNA repair
double-strand break repair
double-strand break repair via nonhomologous end joining
chromatin organization
chromatin remodeling
regulation of transcription, DNA-templated
cellular response to DNA damage stimulus
positive regulation of isotype switching
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
protein homooligomerization
cellular response to X-ray
double-strand break repair via classical nonhomologous end joining
DNA strand resection involved in replication fork processing
negative regulation of DNA strand resection involved in replication fork processing
positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator
protein localization to site of double-strand break
negative regulation of double-strand break repair via homologous recombination
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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