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Identifyn
53BP1 Polyclonal Antibody, Alexa Fluor™ 680, Identifyn™
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图: 1 / 7
53BP1 Antibody (DC-000065) in ICC/IF
Identifyn Detailed protocols can be found below under Documents & Downloads. Widefield: HeLa cells were grown on acid-etched 1.5H cover glass to ~50% confluency, then fixed in -20°C methanol and blocked with Thermo Fisher Fish Serum Blocker (Product # 37527X3). The primary antibody, Rabbit anti-Human 53BP1 Polyclonal - Identifyn SRM Alexa Fluor 680, IgG, was incubated for 1 hour at room temperature at a 1:250 dilution. Cells were washed 5X in PBS and mounted using ProLong Diamond Antifade Mountant with DAPI (Product # P36962). Panel A: Rab... View More
产品信息
DC-000065
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Peptide Sequences between Residues 360 - 410 and 1940 - 1970, referencing Uniprot Q12888 and Gene ID 7158.
偶联物
激发/发射光谱
681/704 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
规格
50 µg
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 10% glycerol
内含物
0.02% sodium azide
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Identifyn™ Primary and Secondary antibodies are extensively validated in Immunofluorescence assays by traditional as well as high resolution acquisition like Widefield SIM, Confocal, Airyscan and STORM Microscopy.
Recommended dilution for traditional microscopy:
Widefield - 1:250-1:1,000
Widefield SIM - 1:250-1:1,000
Confocal - 1:250-1:1,000
Recommended dilution for Super Resolution microscopy:
SIM (Structured Illumination Microscopy) 1:100-1:1,000
Single Molecule STORM (Stochastic Optical Reconstruction Microscopy) - 1:100-1:1,000
Detailed protocols can be found below under Documents & Downloads.
靶标信息
53BP1 (P53-binding protein 1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase. Upon DNA damage, 53BP1 is phosphorylated and localizes to the presumptive sites of damage, specifically, double-strand breaks. 53BP1 may have a role in checkpoint signaling during mitosis, enhances TP53-mediated transcriptional activation, and participates in DNA repair by maintaining genomic stability.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 53BP1; FLJ41424; MGC138366; p53-binding protein 1; TP53-binding protein 1; tumor protein 53-binding protein, 1; tumor suppressor p53-binding protein 1; unnamed protein product
基因别名: 53BP1; p202; p53BP1; TDRD30; TP53BP1
UniProt ID: (Human) Q12888
Entrez Gene ID: (Human) 7158
p53 binding
DNA binding
damaged DNA binding
transcription cofactor activity
transcription coactivator activity
protein binding
telomeric DNA binding
histone binding
sequence-specific DNA binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
ubiquitinated histone binding
histone H4K20me2 reader activity
histone reader activity
histone H4K20me methyltransferase activity
DNA damage checkpoint
DNA repair
double-strand break repair
double-strand break repair via nonhomologous end joining
chromatin organization
chromatin remodeling
regulation of transcription, DNA-templated
cellular response to DNA damage stimulus
positive regulation of isotype switching
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
protein homooligomerization
cellular response to X-ray
double-strand break repair via classical nonhomologous end joining
DNA strand resection involved in replication fork processing
negative regulation of DNA strand resection involved in replication fork processing
positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator
protein localization to site of double-strand break
negative regulation of double-strand break repair via homologous recombination
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