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Invitrogen
53BP1 Polyclonal Antibody
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53BP1 Antibody (PA1-16566) in ICC/IF
Immunofluorescence analysis of TP53-binding protein 1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with 53BP1 Polyclonal Antibody (Product # PA1-16566) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 48... View More
产品信息
PA1-16566
应用
建议稀释比
已发表文章
产品规格
种属反应
Bat,
Bovine,
Dog,
Human,
Mouse,
Rodent,
Rat
已发表种属
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Region between residues 1925 and the C-terminus (residue 1972) of human 53BP1.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
0.02% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Human samples have been tested in Western Blot and ICC/IF, and mouse samples have been tested in ICC/IF only.
Suggested positive control: U205, 293T, and MO59K cell lysates and MEF lysates.
Predicted cross-reactivity based on sequence identity: Chimpazee/Equine/Feline/Gibbon/Gorilla/Hamster/Marmoset/Orangutan/Panda/Porcine/Rabbit/Sheep (100%).
靶标信息
53BP1 (P53-binding protein 1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase. Upon DNA damage, 53BP1 is phosphorylated and localizes to the presumptive sites of damage, specifically, double-strand breaks. 53BP1 may have a role in checkpoint signaling during mitosis, enhances TP53-mediated transcriptional activation, and participates in DNA repair by maintaining genomic stability.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 53BP1; FLJ41424; MGC138366; murine p53-binding protein; p53-binding protein 1; TP53-binding protein 1; transformation related protein 53 binding protein 1; transformation related protein 53 binding-protein 1; tumor protein 53-binding protein, 1; tumor protein p53-binding protein, 1; tumor suppressor p53-binding protein 1
基因别名: 53BP1; m53BP1; p202; p53BP1; TDRD30; TP53; TP53BP1; Trp53bp1
UniProt ID: (Human) Q12888, (Mouse) P70399
Entrez Gene ID: (Dog) 478274, (Human) 7158, (Bovine) 509111, (Rat) 296099, (Mouse) 27223
RNA polymerase II activating transcription factor binding
RNA polymerase II transcription cofactor activity
p53 binding
DNA binding
protein binding
methylated histone binding
molecular_function
damaged DNA binding
telomeric DNA binding
sequence-specific DNA binding
transcription factor binding
DNA metabolism protein
DNA damage checkpoint
double-strand break repair via nonhomologous end joining
transcription, DNA-templated
cellular response to DNA damage stimulus
protein sumoylation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of sequence-specific DNA binding transcription factor activity
cellular response to X-ray
DNA repair
transcription from RNA polymerase II promoter
regulation of transcription, DNA-templated
