CD271 (NGF Receptor) Monoclonal Antibody (ME20.4), Super Bright™ 436, eBioscience™

Product Specific Information

Description: The monoclonal antibody ME20.4 recognizes CD271, a 75 kDa, type I transmembrane protein that belongs to the low-affinity neurotrophin receptor and tumor necrosis factor receptor superfamily. CD271 is expressed by neurons, Schwann cells, melanocytes, mesenchymal stem/stromal cells, neural crest stem cells, astrocytes, oligodendrocytes, and a subset of lymphoid cells including follicular dendritic cells. CD271 binds to the ligands NGF, BDNF, NTF3, and NTF4. CD271 contains a death domain which induces apoptosis and regulates cell growth and proliferation through interactions with TRAF2, TRAF4, TRAF6, PTPN13, and RANBP9.

The ME20.4 monoclonal antibody recognizes human, non-human primate, mouse, rat, sheep, rabbit, cat, dog, and pig CD271.

Applications Reported: This ME20.4 antibody has been reported for use in flow cytometric analysis, western blotting, and microscopy.

Applications Tested: This ME20.4 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated HEK-293T cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

Neurotrophic factors control the survival, differentiation and maintenance of neurons in the peripheral and central nervous systems, and of other neural crest-derived cell types. Developing sympathetic neurons are absolutely dependent upon nerve growth factor (NGF) during the period of target competition in vivo. Antibodies reacting specifically with NGFR p75 are useful tools in the detection and characterization of NGFR p75, to enhance our understanding of a wide range of phenomena in the development, plasticity and repair of the nervous system.