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Lamin B1 Antibody (702972RP647) in ICC/IF
Immunofluorescent analysis of Lamin B1 was performed using 70% confluent HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were stained with Lamin B1 Recombinant Rabbit Monoclonal Antibody (10H34L18), Alexa Fluor™ Plus 647 (Product # 702972RP647, 1:400). Panel a) shows representative images of cells that were stained for detection and localization of Lamin B1. Panel b) shows representative images of ... View More
产品信息
702972RP647
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
10H34L18
抗原
Peptides corresponding to human LAMIN B1 [(aa62-aa79) (aa450-aa467)]
偶联物
激发/发射光谱
658/675 nm
查看光谱
形式
Liquid
浓度
1.0 mg/mL
纯化类型
Protein A
保存液
proprietary buffer, pH 6.8
内含物
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Wet ice
RRID
产品详细信息
Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
Excitation: 658 nm; Emission: 675 nm; Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Lamin B1 is a key member of the nuclear lamina structure, encoded by the LMNB1 gene. It is part of the B-type lamins, ubiquitously expressed across all mammalian cells, providing structural integrity and mechanical support by forming a meshwork along the inner nuclear membrane. Lamin B1 is involved in maintaining the shape and stability of the nucleus, playing a critical role in cellular processes such as genome organization, DNA replication, and transcription regulation. The lamin filaments are type V intermediate filament proteins, enabling the nucleus to withstand mechanical stress and preserve its functionality. Lamin B1's expression and dynamics vary during cell differentiation, influencing gene regulation. Its location within the euchromatin-enriched section of the genome suggests potential roles in chromatin organization and the epithelial-to-mesenchymal transition. Governed by complex interactions with other proteins, lamin B1 is integral to cellular architecture and health, with variations in its levels linked to developmental and disease processes. Recent studies have highlighted the importance of understanding the role and dynamics of lamin B1 in human-induced pluripotent stem cells and germ layer cells, offering insights into differentiation-dependent changes and their implications for nuclear structure.
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生物信息学
蛋白别名: Lamin-B1; MGC111419
基因别名: ADLD; LMN; LMN2; LMNB; LMNB1
UniProt ID: (Human) P20700, (Mouse) P14733
Entrez Gene ID: (Human) 4001, (Mouse) 16906
