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53BP1 Antibody (703580) in ICC/IF
Knockdown of 53BP1 was achieved by transfecting HeLa cells with specific siRNA (Silencer® select Product # s14314 & s14315). Immunofluorescence analysis was performed on HeLa cells (untransfected, panel a-d), transfected with 53BP1 specific siRNA (panel i-l) or non-specific scrambled siRNA (panels e-h). Cells were fixed, permeabilized, and labeled with Anti-53BP1 Recombinant Rabbit Monoclonal Antibody (Product # 703580, 1:100 dilution), followed by Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjuga... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
703580
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
7H14L12
抗原
Protein corresponding to human 53BP1 [aa241-aa440]
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
0.5 mg/mL
规格
100 µg
纯化类型
Protein A
保存液
PBS, pH 7.2-7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
产品详细信息
This antibody is predicted to react with Mouse, Rat, Bovine.
靶标信息
53BP1 (P53-binding protein 1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase. Upon DNA damage, 53BP1 is phosphorylated and localizes to the presumptive sites of damage, specifically, double-strand breaks. 53BP1 may have a role in checkpoint signaling during mitosis, enhances TP53-mediated transcriptional activation, and participates in DNA repair by maintaining genomic stability.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 53BP1; FLJ41424; MGC138366; p53-binding protein 1; tumor protein 53-binding protein, 1; tumor suppressor p53-binding protein 1; unnamed protein product
基因别名: 53BP1; p202; p53BP1; TDRD30
UniProt ID: (Human) Q12888
Entrez Gene ID: (Human) 7158
p53 binding
damaged DNA binding
transcription cofactor activity
transcription coactivator activity
protein binding
telomeric DNA binding
histone binding
sequence-specific DNA binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
ubiquitinated histone binding
histone H4K20me2 reader activity
histone reader activity
histone H4K20me methyltransferase activity
DNA damage checkpoint
double-strand break repair via nonhomologous end joining
chromatin organization
chromatin remodeling
regulation of transcription, DNA-templated
cellular response to DNA damage stimulus
positive regulation of isotype switching
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
protein homooligomerization
double-strand break repair via classical nonhomologous end joining
positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator
protein localization to site of double-strand break
negative regulation of double-strand break repair via homologous recombination
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