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GM130 Antibody (703794RP488) in ICC/IF
Immunofluorescence analysis of GM130 was performed using GM130 Recombinant Rabbit Monoclonal antibody (10H5L5), Alexa Fluor™ Plus 488 (Product # 703794RP488, 1:400) on 70-80% confluent HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were stained with GM130 Recombinant Rabbit Monoclonal antibody (10H5L5), Alexa Fluor™ Plus 488 (Product # 703794RP488). Panel a) shows representative image of cells tha... View More
Product Details
703794RP488
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
10H5L5
Conjugate
Excitation/Emission Max
493/518 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
proprietary buffer, pH 6.3-7.3
Contains
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
Product Specific Information
Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 494 nm; Emission: 519 nm; Laser: Blue Laser
Target Information
The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This encoded protein has been postulated to play roles in the stacking of Golgi cisternae and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 130 kDa cis-Golgi matrix protein; GM130; GM130 autoantigen; golgi autoantigen, golgin subfamily a, 2; Golgi matrix protein GM130; Golgin subfamily A member 2; Golgin-95; RP11-395P17.5; SY11 protein
Gene Aliases: GM130; GOLGA2
UniProt ID: (Human) Q08379
Entrez Gene ID: (Human) 2801
protein glycosylation
ER to Golgi vesicle-mediated transport
microtubule nucleation
asymmetric cell division
negative regulation of autophagy
negative regulation of protein binding
COPII vesicle coating
spindle assembly
protein homotetramerization
centrosome organization
positive regulation of protein glycosylation
Golgi ribbon formation
Golgi disassembly
spindle assembly involved in meiosis
mitotic spindle assembly
cell-cell adhesion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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