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CD4 Antibody (740028TP488) in ICC/IF
Immunofluorescent analysis of CD4 was performed using CD4 transfected HEK-293E cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were stained with CD4 Recombinant Rat Monoclonal Antibody (4SM95), Alexa Fluor™ Plus 488 (Product # 740028TP488, 1:800). Panel a) shows representative images of cells that were stained for detection and localization of CD4. Panel b) shows representative images of cells stained f... View More
产品信息
740028TP488
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rat
/ IgG1, kappa
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
4SM95
偶联物
激发/发射光谱
493/518 nm
查看光谱
形式
Liquid
浓度
1.0 mg/mL
纯化类型
Affinity chromatography
保存液
proprietary buffer, pH 6.8
内含物
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Wet ice
RRID
产品详细信息
Alexa Fluor™ Plus recombinant rat antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
Applications Tested: This 4SM95 antibody has been tested by immunohistochemistry of colon and immunocytochemistry of CD4 transfected HEK-293E cells and flow cytometric analysis of human PBMC. This may be used for immunohistochemistry at 10 µg/mL and for immunocytochemistry at 1.25 µg/mL and for flow cytometry at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 494 nm; Emission: 519 nm; Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CD4; CD4 antigen (p55); CD4 antigen p55; CD4 receptor; cd4a; fCD4; Leu-3; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4)
基因别名: CD4; CD4mut; L3T4; Ly-4
UniProt ID: (Human) P01730, (Mouse) P06332
Entrez Gene ID: (Human) 920, (Mouse) 12504
virus receptor activity
glycoprotein binding
receptor activity
transmembrane signaling receptor activity
extracellular matrix structural constituent
protein binding
zinc ion binding
coreceptor activity
immunoglobulin binding
enzyme binding
protein kinase binding
MHC class II protein binding
protein homodimerization activity
protein tyrosine kinase binding
cytokine production
immune system process
induction by virus of host cell-cell fusion
immune response
cell adhesion
cell surface receptor signaling pathway
T cell differentiation
maintenance of protein location in cell
T cell activation
T cell selection
protein palmitoleylation
positive regulation of protein kinase activity
positive regulation of peptidyl-tyrosine phosphorylation
defense response to Gram-negative bacterium
positive regulation of calcium-mediated signaling
regulation of T cell activation
positive regulation of T cell activation
adaptive immune response
signal transduction
enzyme linked receptor protein signaling pathway
transmembrane receptor protein tyrosine kinase signaling pathway
positive regulation of calcium ion transport into cytosol
fusion of virus membrane with host plasma membrane
entry into host cell
T cell costimulation
response to estradiol
response to vitamin D
helper T cell enhancement of adaptive immune response
positive regulation of T cell proliferation
positive regulation of interleukin-2 biosynthetic process
regulation of defense response to virus by virus
T cell receptor signaling pathway
