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FIGURE: 1 / 6
OCT3/4 Antibody (740044T) in ICC/IF
Immunofluorescent analysis of OCT3/4 was performed using 70% confluent NTERA-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were stained with OCT3/4 Recombinant Rat Monoclonal Antibody (EM92) (Product # 740044T, 1:200) at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor™ Plus 647 (Product # A32728, 1:2,000) for 1 hour at room temperature. Pane... View More
Product Details
740044T
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rat
/ IgG2, kappa
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
EM92
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
Recombinant Rat Monoclonal Antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Target Information
POU5F1, also commonly known as Oct-4, is a maternally expressed octamer-binding protein that was the first transcription factor described for the early stages of development. The role of POU5F1 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing POU5F1, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 3-Oct; 4-Oct; DADB-104B20.2; gp-9; HGNC:9221; MGC22487; NF-A3; Oct-3; Oct-4; OCT4-B1; OCT4B-variant 2; Octamer-binding protein 3; Octamer-binding protein 4; Octamer-binding transcription factor 3; OTF-3; OTTHUMP00000221150; OTTHUMP00000221151; POU class 5 homeobox 1; POU domain transcription factor OCT4; POU domain, class 5, transcription factor 1; pou-2; POU-type homeodomain-containing DNA-binding protein; pou2; transcriptional regulator
Gene Aliases: NF-A3; Oct-3; Oct-3/4; Oct-4; OCT3; Oct3/4; OCT4; OCT4Borf1; OTF-3; Otf-4; OTF3; Otf3-rs7; Otf3g; OTF4; POU5F1
UniProt ID: (Human) Q01860, (Mouse) P20263
Entrez Gene ID: (Human) 5460, (Mouse) 18999
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
RNA binding
protein binding
ubiquitin protein ligase binding
miRNA binding
sequence-specific DNA binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
sequence-specific double-stranded DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II transcription coactivator activity
chromatin binding
transcription corepressor activity
transcription factor binding
chromatin DNA binding
transcription regulatory region DNA binding
poly(A) RNA binding
protein heterodimerization activity
POU domain binding
HMG box domain binding
negative regulation of transcription from RNA polymerase II promoter
endodermal cell fate specification
blastocyst development
heart induction
endodermal-mesodermal cell signaling
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
response to wounding
anatomical structure morphogenesis
regulation of asymmetric cell division
regulation of gene expression
BMP signaling pathway
somatic stem cell population maintenance
positive regulation of transcription from RNA polymerase II promoter
cell fate commitment involved in formation of primary germ layer
cardiac cell fate determination
positive regulation of canonical Wnt signaling pathway
negative regulation of pri-miRNA transcription from RNA polymerase II promoter
mesodermal cell fate commitment
endodermal cell fate commitment
ectodermal cell fate commitment
trophectodermal cell differentiation
blastocyst growth
BMP signaling pathway involved in heart induction
transcription, DNA-templated
transcription from RNA polymerase II promoter
multicellular organism development
response to organic substance
stem cell population maintenance
germ-line stem cell population maintenance
response to retinoic acid
positive regulation of catenin import into nucleus
mRNA transcription from RNA polymerase II promoter
cell fate commitment
negative regulation of cell differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
negative regulation of calcium ion-dependent exocytosis
stem cell differentiation
positive regulation of protein kinase B signaling
negative regulation of protein kinase B signaling
positive regulation of SMAD protein import into nucleus
negative regulation of gene silencing by miRNA
regulation of heart induction by regulation of canonical Wnt signaling pathway
regulation of methylation-dependent chromatin silencing
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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