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Invitrogen

Phospho-BTK/ITK (Tyr551, Tyr511) Recombinant Mouse Monoclonal Antibody (M4G3LN), Alexa Fluor™ Plus 647

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Product Details

740047MP647

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

0.25 µg/test
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2b, kappa

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

M4G3LN

Conjugate

Alexa Fluor™ Plus 647 Alexa Fluor™ Plus 647 Alexa Fluor™ Plus 647

Excitation/Emission Max

658/675 nm View spectra spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A/G

Storage buffer

proprietary buffer, pH 6.3-7.3

Contains

0.008% Bromonitrodioxane, 0.008% Methylisothiazolone

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Wet ice

RRID

AB_3699404

Product Specific Information

Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.

Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 658 nm; Emission: 675 nm; Laser: Red Laser

Target Information

In addition to Src family and SYK family kinases, B cells and T cells express a third family of receptor-proximal kinases called the Tec kinase family. The B cell-specific Tec family kinase is BTK; T cells express ITK as well as Tec and Rlk. Tec family kinases play an indispensable role in activation of phospholipase C and activation of calcium-dependent signaling cascades following antigen receptor triggering. BTK has been implicated in propagating signals downstream of LPS-TLR4 in B cells via signaling through the TLR4 co-receptor RP105/CD180. Mice congenitally lacking BTK or ITK show profound defects in B cell or T cell development and signaling, respectively. People with mutations or deletions in BTK suffer from X-linked agammaglobulinemia and X-linked immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: agammaglobulinaemia tyrosine kinase; Agammaglobulinemia tyrosine kinase; ATK; B-cell progenitor kinase; BPK; Bruton agammaglobulinemia tyrosine kinase; Bruton tyrosine kinase; Bruton's tyrosine kinase; dominant-negative kinase-deficient Brutons tyrosine kinase; frameshift mutation results in truncated protein; homolog of mouse T-cell itk/tsk; IL-2-inducible T-cell kinase; Interleukin-2-inducible T-cell kinase; Kinase EMT; T-cell-specific kinase; Tyrosine-protein kinase BTK; Tyrosine-protein kinase ITK/TSK; Tyrosine-protein kinase Lyk; unnamed protein product

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Gene Aliases: AGMX1; AT; ATK; BPK; BTK; EMT; IGHD3; IMD1; ITK; LPFS1; LYK; PSCTK1; PSCTK2; XLA

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UniProt ID: (Human) Q06187, (Human) Q08881

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Entrez Gene ID: (Human) 695, (Human) 3702

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Function(s)
nucleotide binding protein kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity protein binding ATP binding phosphatidylinositol-3,4,5-trisphosphate binding zinc ion binding lipid binding phospholipase activator activity kinase activity transferase activity identical protein binding phospholipase binding metal ion binding
Process(es)
neutrophil homeostasis positive regulation of type III hypersensitivity positive regulation of type I hypersensitivity negative regulation of cytokine production adaptive immune response B cell affinity maturation immune system process histamine secretion by mast cell positive regulation of immunoglobulin production regulation of B cell cytokine production MyD88-dependent toll-like receptor signaling pathway regulation of B cell apoptotic process apoptotic process mesoderm development peptidyl-tyrosine phosphorylation calcium-mediated signaling proteoglycan catabolic process negative regulation of B cell proliferation positive regulation of B cell proliferation positive regulation of type I interferon production response to lipopolysaccharide negative regulation of interleukin-10 production positive regulation of interleukin-6 production positive regulation of tumor necrosis factor production cellular response to reactive oxygen species intracellular signal transduction Fc-epsilon receptor signaling pathway B cell activation positive regulation of I-kappaB kinase/NF-kappaB signaling innate immune response positive regulation of B cell differentiation cell maturation positive regulation of phagocytosis T cell receptor signaling pathway B cell receptor signaling pathway regulation of B cell activation negative regulation of B cell activation positive regulation of NF-kappaB transcription factor activity monocyte proliferation negative regulation of leukocyte proliferation cellular response to molecule of fungal origin apoptotic signaling pathway cellular response to interleukin-7 cGAS/STING signaling pathway positive regulation of cGAS/STING signaling pathway positive regulation of interleukin-17A production positive regulation of NLRP3 inflammasome complex assembly positive regulation of synoviocyte proliferation eosinophil homeostasis positive regulation of cytokine production NK T cell differentiation cellular defense response signal transduction T cell activation gamma-delta T cell activation
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