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Phospho-4EBP1 (Thr36, Thr45) Recombinant Mouse Monoclonal Antibody (V3NTY24), Alexa Fluor™ Plus 555
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FIGURE: 1 / 2
Phospho-4EBP1 (Thr36, Thr45) Antibody (740048MP555) in Flow
Flow cytometry analysis of Phospho-4EBP1 (Thr36, Thr45) Recombinant Mouse Monoclonal Antibody (V3NTY24), Alexa Fluor™ Plus 555 (Product # 740048MP555) was performed using Serum Starved MV4-11 T27cells untreated and treated with Pervanadate (1mM for 30 minutes). Cells were fixed with eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set (Product # 00-5523-00), permeabilized and stained with 1 µg of P... View More
Product Details
740048MP555
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b, kappa
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
V3NTY24
Conjugate
Alexa Fluor™ Plus 555
Alexa Fluor™ Plus 555
Alexa Fluor™ Plus 555
Excitation/Emission Max
558/572 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
50 µg
Purification
Protein A/G
Storage buffer
proprietary buffer, pH 6.3-7.3
Contains
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
Product Specific Information
Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 553 nm; Emission: 568 nm; Laser: Yellow Laser
Target Information
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E, which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 4E-BP1; eIF4E-binding protein 1; EIF4EBP1; Eukaryotic translation initiation factor 4E-binding protein 1; MGC431; MGC4316; P/OKCL.6; PHAS-1; PHAS-I; Phosphorylated heat- and acid-stable protein regulated by insulin 1; unnamed protein product
Gene Aliases: 4E-BP1; 4EBP1; BP-1; EIF4EBP1; PHAS-I
UniProt ID: (Human) Q13541
Entrez Gene ID: (Human) 1978
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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