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Invitrogen

Phospho AKT1 (Ser473) Recombinant Mouse Monoclonal Antibody (SDRNR), Alexa Fluor™ Plus 647

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Product Details

740063MP647

Applications
Tested Dilution
Publications

Western Blot (WB)

1:200
-

Flow Cytometry (Flow)

2 µg/test
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a, kappa

Expression System

ExpiCHO

Class

Recombinant Monoclonal

Type

Antibody

Clone

SDRNR

Conjugate

Alexa Fluor™ Plus 647 Alexa Fluor™ Plus 647 Alexa Fluor™ Plus 647

Excitation/Emission Max

658/675 nm View spectra spectra

Form

Liquid

Concentration

1 mg/mL

Amount

50 µg

Purification

Protein A/G

Storage buffer

proprietary buffer, pH 6.3-7.3

Contains

0.008% Bromonitrodioxane, 0.008% Methylisothiazolone

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Wet ice

Product Specific Information

Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.

Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 658 nm; Emission: 675 nm; Laser: Red Laser

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

AKT1 (PKB alpha) is a serine/threonine kinase that regulates cell survival. The activated enzyme inhibits apoptosis and stimulates cell cycle progression by phosphorylating numerous targets in various cell types, including cancer cells. This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. The AKT pathway is a major target for cancer drug discovery.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: AKT; AKT1M; C-AKT; CAKT; PKB ALPHA; PKB beta; PKB gamma; PKB-ALPHA; PKBG; PRKBA; PROTEIN KINASE B ALPHA; PROTO-ONCOGENE C-AKT; RAC; rac protein kinase alpha; RAC-ALPHA; RAC-ALPHA SERINE/THREONINE-PROTEIN KINASE; RAC-PK-ALPHA; RAC-PK-beta; RAC-PK-gamma; serine-threonine protein kinase; unnamed protein product; V-AKT MURINE THYMOMA VIRAL ONCOGENE HOMOLOG 1; V-AKT MURINE THYMOMA VIRAL ONCOGENE-LIKE PROTEIN 1

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Gene Aliases: AKT; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA

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UniProt ID: (Human) P31749

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Entrez Gene ID: (Human) 207

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Function(s)
protein kinase activity protein serine/threonine kinase activity protein serine/threonine/tyrosine kinase activity protein binding calmodulin binding ATP binding phosphatidylinositol-3,4,5-trisphosphate binding kinase activity enzyme binding kinase binding protein kinase binding nitric-oxide synthase regulator activity protein serine/threonine kinase inhibitor activity identical protein binding protein homodimerization activity phosphatidylinositol-3,4-bisphosphate binding 14-3-3 protein binding potassium channel activator activity protein serine kinase activity TORC2 complex binding
Process(es)
osteoblast differentiation positive regulation of endothelial cell proliferation cell migration involved in sprouting angiogenesis complement receptor mediated signaling pathway sphingosine-1-phosphate signaling pathway regulation of glycogen biosynthetic process protein phosphorylation nitric oxide biosynthetic process activation-induced cell death of T cells response to oxidative stress signal transduction epidermal growth factor receptor signaling pathway G-protein coupled receptor signaling pathway cell proliferation insulin receptor signaling pathway response to heat response to hormone negative regulation of autophagy positive regulation of endothelial cell migration positive regulation of gene expression negative regulation of gene expression negative regulation of plasma membrane long-chain fatty acid transport fibroblast migration positive regulation of glucose metabolic process regulation of neuron projection development negative regulation of macroautophagy peptidyl-serine phosphorylation peptidyl-threonine phosphorylation cytokine-mediated signaling pathway cell differentiation positive regulation of cell growth regulation of cell migration positive regulation of cell migration T cell costimulation negative regulation of protein ubiquitination TOR signaling negative regulation of fatty acid beta-oxidation positive regulation of endodeoxyribonuclease activity positive regulation of proteasomal ubiquitin-dependent protein catabolic process cellular response to insulin stimulus positive regulation of peptidyl-serine phosphorylation cellular response to stress response to fluid shear stress intracellular signal transduction interleukin-18-mediated signaling pathway positive regulation of protein import into nucleus regulation of apoptotic process negative regulation of apoptotic process anoikis regulation of mRNA stability protein kinase B signaling positive regulation of blood vessel endothelial cell migration positive regulation of nitric oxide biosynthetic process positive regulation of fat cell differentiation positive regulation of glycogen biosynthetic process negative regulation of Notch signaling pathway negative regulation of proteolysis positive regulation of transcription from RNA polymerase II promoter nitric oxide metabolic process positive regulation of glucose import positive regulation of lipid biosynthetic process insulin-like growth factor receptor signaling pathway behavioral response to pain positive regulation of smooth muscle cell proliferation positive regulation of protein metabolic process positive regulation of protein kinase B signaling excitatory postsynaptic potential response to growth hormone mammary gland epithelial cell differentiation response to UV-A protein localization to mitochondrion response to growth factor cellular response to epidermal growth factor stimulus maintenance of protein location in mitochondrion cellular response to rapamycin negative regulation of release of cytochrome c from mitochondria vascular endothelial cell response to laminar fluid shear stress regulation of postsynapse organization regulation of tRNA methylation cellular response to oxidised low-density lipoprotein particle stimulus negative regulation of protein localization to lysosome negative regulation of cGAS/STING signaling pathway beta-arrestin-dependent dopamine receptor signaling pathway positive regulation of G1/S transition of mitotic cell cycle positive regulation of protein localization to nucleus regulation of signal transduction by p53 class mediator negative regulation of cilium assembly negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway negative regulation of leukocyte cell-cell adhesion positive regulation of protein localization to plasma membrane negative regulation of protein maturation negative regulation of hydrogen peroxide-induced neuron intrinsic apoptotic signaling pathway negative regulation of PERK-mediated unfolded protein response positive regulation of TORC1 signaling positive regulation of TORC2 signaling positive regulation of protein localization to endoplasmic reticulum positive regulation of anaphase-promoting complex-dependent catabolic process cellular response to nerve growth factor stimulus response to insulin-like growth factor stimulus positive regulation of protein localization to cell surface regulation of type B pancreatic cell development negative regulation of lymphocyte migration negative regulation of extrinsic apoptotic signaling pathway in absence of ligand negative regulation of intrinsic apoptotic signaling pathway
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