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IkB zeta Antibody (740065T) in ICC/IF
Immunofluorescent analysis of IκB Zeta was performed using 70% confluent RAW 264.7 cells with or without LPS treatment (1 µg/mL, 6 hours). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were stained with IκB Zeta Recombinant Rat Monoclonal Antibody (LK2NAP) (Product # 740065T, 1:200). Panels a) and e) shows representative images of cells that were stained for detection and localization of IκB Zeta. Panels b) ... View More
Product Details
740065T
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rat
/ IgG2a
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
LK2NAP
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
Recombinant rat monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Target Information
I-kappa-B-zeta (also known as MAIL and INAP) is an ankyrin-repeat-containing nuclear protein that is highly homologous to the IkappaB family member Bcl-3. Transcription of IkappaBzeta is upregulated by stimulation with TLR ligands, IL-1 and IL-6 in cultured B-lymphocytes and monocytes/macrophages, but only faintly so in T-lymphocytes, fibroblasts, and endothelial cells. IkappaB-zeta preferentially associates with the NF-kappaB subunit p50 rather than p65 and recombinant IkappaB-zeta proteins inhibit the DNA binding of the p65/p50 heterodimer and the p50/p50 homodimer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ30225; FLJ34463; I-kappa-B-zeta; Ikappa B-zeta variant 3; IkappaB-zeta; ikappaBzeta; IkB-zeta; IL-1 inducible nuclear ankyrin-repeat protein; INAP; MAIL; Molecule possessing ankyrin repeats induced by lipopolysaccharide; molecule possessing ankyrin-repeats induced by lipopolysaccharide; NF-kappa-B inhibitor zeta; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, zeta; OTTHUMP00000214340
Gene Aliases: AA408868; IKBZ; INAP; MAIL; NFKBIZ; RGD1310834
UniProt ID: (Human) Q9BYH8, (Mouse) Q9EST8
Entrez Gene ID: (Human) 64332, (Rat) 304005, (Mouse) 80859
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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