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Invitrogen

Phospho-STAT3 (Tyr705) Recombinant Mouse Monoclonal Antibody (LUVNKLA), Alexa Fluor™ Plus 647

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Product Details

740067MP647

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

0.25 µg/test
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Mouse / IgG2b, kappa

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

LUVNKLA

Conjugate

Alexa Fluor™ Plus 647 Alexa Fluor™ Plus 647 Alexa Fluor™ Plus 647

Excitation/Emission Max

658/675 nm View spectra spectra

Form

Liquid

Concentration

1.0 mg/mL

Amount

50 µg

Purification

Protein A/G

Storage buffer

proprietary buffer, pH 6.8

Contains

0.008% Bromonitrodioxane, 0.008% Methylisothiazolone

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Wet ice

RRID

AB_3679209

Product Specific Information

Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.

Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.

Applications Tested: This LUVNKLA antibody has been tested by flow cytometric analysis of Jurkat cells. This may be used for flow cytometry at 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 658 nm; Emission: 675 nm; Laser: Red Laser

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

STAT3 belongs to the family of STAT (signal transducers and activators of transcription) proteins which are phosphorylated by receptor associated kinases, translocate to the nucleus, and act as transcription factors in response to cytokines and growth factors. Coactivators such as CREB-binding protein are required for the transcriptional activation by STAT3. STAT3 can also be activated by Interferon-alpha, Interferon-gamma, EGF, PDGF and IL6. Phosphorylation on tyrosine 705 by JAK1 and JAK2 is essential for STAT3 dimer formation, nuclear translocation, and DNA binding activity. In humans, the STAT3 gene is located on the q arm of chromosome 17. STAT3 has been shown to be activated by IFN-alpha but not IFN-beta. The transcription factors associated with STAT3 are c-Jun and cyclic AMP-responsive enhancer binding protein (CREB). STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3 while the PIAS3 protein is a specific inhibitor of STAT3. Three alternatively spliced transcript variants encoding distinct isoforms of STAT3 have been described. Deletion of the STAT3 gene in knock-out mice was lethal at the early embryonic stage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: acute phase response factor; Acute-phase response factor; DNA-binding protein APRF; FLJ20882; MGC16063; Signal transducer and activator of transcription 3; STAT; Stat3; unnamed protein product

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Gene Aliases: 1110034C02Rik; ADMIO; ADMIO1; APRF; AW109958; HIES; STAT3

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UniProt ID: (Human) P40763, (Mouse) P42227

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Entrez Gene ID: (Human) 6774, (Mouse) 20848

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Function(s)
transcription regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding RNA binding RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding receptor binding protein binding protein kinase binding protein phosphatase binding chromatin DNA binding CCR5 chemokine receptor binding glucocorticoid receptor binding signaling adaptor activity identical protein binding protein homodimerization activity sequence-specific DNA binding protein dimerization activity RNA polymerase II sequence-specific DNA binding transcription factor binding primary miRNA binding lncRNA binding DNA-binding transcription factor binding protein sequestering activity RNA sequestering activity RNA polymerase II repressing transcription factor binding signal transducer activity transcription factor binding transcription regulatory region DNA binding DNA-binding transcription factor
Process(es)
negative regulation of transcription from RNA polymerase II promoter temperature homeostasis response to hypoxia eye photoreceptor cell differentiation regulation of cytokine production response to ischemia regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter protein import into nucleus defense response acute-phase response inflammatory response immune response signal transduction transforming growth factor beta receptor signaling pathway I-kappaB kinase/NF-kappaB signaling JAK-STAT cascade nervous system development cell proliferation positive regulation of cell proliferation negative regulation of cell proliferation gene expression negative regulation of autophagy positive regulation of vascular endothelial growth factor production positive regulation of gene expression negative regulation of gene expression negative regulation of hydrogen peroxide biosynthetic process phosphorylation cytokine-mediated signaling pathway sexual reproduction signal transduction involved in regulation of gene expression cell differentiation positive regulation of cell migration intracellular receptor signaling pathway response to estradiol positive regulation of interleukin-1 beta production positive regulation of interleukin-10 production positive regulation of interleukin-6 production positive regulation of interleukin-8 production positive regulation of tumor necrosis factor production cellular response to hormone stimulus leptin-mediated signaling pathway response to cytokine somatic stem cell population maintenance interleukin-15-mediated signaling pathway interleukin-2-mediated signaling pathway interleukin-9-mediated signaling pathway interleukin-11-mediated signaling pathway interleukin-23-mediated signaling pathway regulation of multicellular organism growth regulation of cell proliferation glucose homeostasis eating behavior mRNA transcription from RNA polymerase II promoter positive regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of I-kappaB kinase/NF-kappaB signaling response to peptide hormone protein kinase B signaling cellular response to leptin stimulus response to leptin response to ethanol positive regulation of erythrocyte differentiation positive regulation of Notch signaling pathway positive regulation of angiogenesis negative regulation of glycolytic process positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter regulation of mitochondrial membrane permeability astrocyte differentiation negative regulation of inflammatory response positive regulation of inflammatory response positive regulation of phagocytosis modulation of synaptic transmission positive regulation of multicellular organismal process regulation of cell cycle radial glial cell differentiation retinal rod cell differentiation regulation of feeding behavior growth hormone receptor signaling pathway JAK-STAT cascade involved in growth hormone signaling pathway interleukin-6-mediated signaling pathway cellular response to cytokine stimulus T-helper 17 type immune response T-helper 17 cell lineage commitment positive regulation of extracellular matrix disassembly inflammatory response to wounding energy homeostasis cellular response to interleukin-17 receptor signaling pathway via STAT postsynapse to nucleus signaling pathway negative regulation of inflammatory response to wounding interleukin-10-mediated signaling pathway negative regulation of cytokine production involved in inflammatory response positive regulation of cytokine production involved in inflammatory response regulation of cellular response to hypoxia positive regulation of growth factor dependent skeletal muscle satellite cell proliferation positive regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of vascular endothelial cell proliferation negative regulation of 3'-UTR-mediated mRNA stabilization negative regulation of primary miRNA processing negative regulation of stem cell differentiation positive regulation of ATP biosynthetic process negative regulation of neuron migration transcription, DNA-templated stem cell population maintenance negative regulation of apoptotic process negative regulation of cell death negative regulation of neuron death
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