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CXCL10 (IP-10) Antibody (740068MP647) in Flow
Flow cytometry analysis of CXCL10 Recombinant Mouse Monoclonal Antibody (4NY8UN), Alexa Fluor™ Plus 647 (Product # 740068MP647) was performed using Human PBMCs untreated and treated with IFN gamma (Product # PHC4031, 50 ng/mL for 5 hours). Human PBMCs were stained with CD14 Monoclonal Antibody (61D3), eFluor™ 450, eBioscience™ (Product # 48-0149-42) and 1 µg of CXCL10 Recombinant Mouse Monoclonal Antibody (4NY8UN), Alexa Fluor™ Plus 647 (Product # 740068MP647). An increased expression of CXCL10 was observed in treated PBMCs (right dot plot... View More
Product Details
740068MP647
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b, kappa
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
4NY8UN
Conjugate
Excitation/Emission Max
658/675 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A/G
Storage buffer
proprietary buffer, pH 6.3-7.3
Contains
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
Product Specific Information
Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 658 nm; Emission: 675 nm; Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
IP-10 (interferon-gamma induced protein 10 kD; in mouse, also called cytokine responsive gene 2, Crg-2, or mob-1) is a highly inducible, primary response gene that belongs to the CXC chemokine superfamily. It was first cloned in 1985. The biological functions of IP-10 are still unclear. Like Mig, IP-10 has no activity on neutrophils. Its functions include stimulation of monocytes, natural killer and T-cell migration, regulation of T-cell and bone marrow progenitor maturation, modulation of adhesion molecule expression as well as inhibition of angiogenesis. Mig and IP-10 share the same receptor, CXCR3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 10 kDa interferon gamma-induced protein; C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 10; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 10; CXC; CXC motif chemokine; CXCL; gamma IP10; Gamma-IP10; H-IP-10; interferon-inducible cytokine IP-10; M-IP-10; protein 10 from interferon (gamma)-induced cell line; small inducible cytokine subfamily B (Cys-X-Cys), member 10; Small-inducible cytokine B10
Gene Aliases: C7; crg-2; CXCL10; gIP-10; IFI10; INP10; IP-10; mob-1; SCYB10
UniProt ID: (Human) P02778
Entrez Gene ID: (Human) 3627
chemotaxis
inflammatory response
immune response
signal transduction
cell surface receptor signaling pathway
G-protein coupled receptor signaling pathway
cell-cell signaling
muscle organ development
blood circulation
positive regulation of cell proliferation
response to cold
response to gamma radiation
T cell chemotaxis
regulation of T cell chemotaxis
response to auditory stimulus
negative regulation of angiogenesis
positive regulation of cAMP metabolic process
response to vitamin D
cellular response to heat
endothelial cell activation
regulation of cell proliferation
positive regulation of cAMP-mediated signaling
negative regulation of myoblast differentiation
regulation of protein kinase activity
positive regulation of transcription from RNA polymerase II promoter
positive regulation of release of sequestered calcium ion into cytosol
defense response to virus
chemokine-mediated signaling pathway
cellular response to lipopolysaccharide
positive regulation of monocyte chemotaxis
regulation of endothelial tube morphogenesis
negative regulation of myoblast fusion
positive regulation of T cell migration
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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