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Invitrogen

Insulin Recombinant Mouse Monoclonal Antibody (ICBTACLS), Alexa Fluor™ Plus 488

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Product Details

740072MP488

Applications
Tested Dilution
Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50
-

Immunocytochemistry (ICC/IF)

1:200
-

Flow Cytometry (Flow)

0.125 µg/test
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a, kappa

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

ICBTACLS

Conjugate

Alexa Fluor™ Plus 488 Alexa Fluor™ Plus 488 Alexa Fluor™ Plus 488

Excitation/Emission Max

493/518 nm View spectra spectra

Form

Liquid

Concentration

1 mg/mL

Amount

50 µg

Purification

Protein A/G

Storage buffer

proprietary buffer, pH 6.3-7.3

Contains

0.008% Bromonitrodioxane, 0.008% Methylisothiazolone

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Wet ice

Product Specific Information

Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.

Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 494 nm; Emission: 519 nm; Laser: Blue Laser

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Insulin is a peptide hormone secreted by beta cells of the pancreatic islets. It regulates carbohydrate, protein and lipid metabolism by enhancing membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Insulin is 51-amino acid polypeptide product produced from a precursor peptide, proinsulin. Proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds and one molecule of C-peptide. Insulin deficiency results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of b-cell origin such as insulinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Ins; INSU1; INSU2; INSU3B; INSUA; INSUB; INSUC; Insulin; insulin splice variant; insulin variant; INS-splice; Insulin-1; preproinsulin; proinsulin

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Gene Aliases: IDDM; IDDM1; IDDM2; ILPR; INS; IRDN; MODY10; PNDM4

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UniProt ID: (Human) P01308

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Entrez Gene ID: (Human) 3630

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Function(s)
protease binding insulin receptor binding insulin-like growth factor receptor binding hormone activity protein binding identical protein binding receptor agonist activity
Process(es)
positive regulation of cytokine production negative regulation of acute inflammatory response glucose metabolic process regulation of transcription, DNA-templated acute-phase response G-protein coupled receptor signaling pathway cell-cell signaling positive regulation of cell proliferation insulin receptor signaling pathway positive regulation of gene expression negative regulation of gene expression positive regulation of nitric oxide mediated signal transduction positive regulation of neuron projection development positive regulation of cell growth positive regulation of cell migration regulation of protein localization nitric oxide-cGMP-mediated signaling pathway TORC1 signaling wound healing negative regulation of protein catabolic process vasodilation glucose homeostasis positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of MAPK cascade positive regulation of cell differentiation negative regulation of gluconeogenesis positive regulation of glycogen biosynthetic process positive regulation of translation negative regulation of glycogen catabolic process positive regulation of glycolytic process positive regulation of mitotic nuclear division negative regulation of fatty acid metabolic process positive regulation of glucose import positive regulation of insulin receptor signaling pathway alpha-beta T cell activation positive regulation of lipid biosynthetic process regulation of synaptic plasticity regulation of protein secretion negative regulation of protein secretion positive regulation of protein secretion cognition negative regulation of lipid catabolic process positive regulation of nitric-oxide synthase activity positive regulation of protein kinase B signaling fatty acid homeostasis negative regulation of respiratory burst involved in inflammatory response positive regulation of respiratory burst positive regulation of peptide hormone secretion positive regulation of brown fat cell differentiation positive regulation of protein localization to nucleus positive regulation of long-term synaptic potentiation cellular response to oxygen-containing compound negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway positive regulation of dendritic spine maintenance regulation of protein localization to plasma membrane negative regulation of reactive oxygen species biosynthetic process neuron projection maintenance negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process negative regulation of feeding behavior
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