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Invitrogen
CD133 (Prominin-1) Recombinant Mouse Monoclonal Antibody (EMK08)
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FIGURE: 1 / 6
CD133 (Prominin-1) Antibody (740104M) in ICC/IF
Immunofluorescence analysis of CD133 was performed using CD133 Recombinant Mouse Monoclonal Antibody (EMK08) (Product # 740104M, 1:200) on 70-80% confluent HT-29 and HeLa cells lines. The cell lines were fixed with 4% paraformaldehyde for 10 minutes and then blocked with 2% BSA for 1 hour at room temperature. The cells were stained with CD133 Recombinant Mouse Monoclonal Antibody (EMK08) in 0.1% BSA, incuba... View More
Product Details
740104M
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b, kappa
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
EMK08
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A/G
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Recombinant mouse monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Target Information
The CD133 (AC133) antigen is a 97 kDa glycoprotein, with 5 transmembrane domains, that shows up as a 120 kDa band on protein gels due to glycosylation. CD133 is a cell surface marker expressed by immature hematopoietic stem cells but not mature blood cells. In addition to hematopoietic stem and progenitor cells, CD133 has been found to be a marker for other stem and progenitor cells including neural and embryonic stem cells. CD133 has been shown to be expressed in cancers, including some leukemias and brain tumors, that may be derived from stem cells. Low level expression could also be detected in the kidney, pancreas, placenta, and fetal liver. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of the CD133 gene is also associated with several types of cancer. CD133 is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner, and multiple transcript variants encoding different isoforms have been found.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Antigen AC133; antigen CD133; CD107b; CD133; hematopoietic stem cell antigen; hProminin; Lamp II; LAMP-2; Lamp-2a; Lamp-2b; Lamp-2c; Lamp2; LGP-96; LGP-B; Mac3; Prominin-1; Prominin-like protein 1; Prominin1
Gene Aliases: AC133; CD133; CORD12; MCDR2; MSTP061; PROM1; PROML1; RP41; STGD4
UniProt ID: (Human) O43490
Entrez Gene ID: (Human) 8842
retina layer formation
photoreceptor cell maintenance
retina morphogenesis in camera-type eye
camera-type eye photoreceptor cell differentiation
glomerular visceral epithelial cell differentiation
glomerular parietal epithelial cell differentiation
positive regulation of nephron tubule epithelial cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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