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Invitrogen
EOMES Recombinant Mouse Monoclonal Antibody (WD1928), Alexa Fluor™ Plus 488
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EOMES Antibody (740127MP488) in Flow
Flow cytometry analysis of EOMES Recombinant Mouse Monoclonal Antibody (WD1928), Alexa Fluor ™ Plus 488 (Product # 740127MP488) was performed using NK-92 and THP-1 cells. Cells were stained with 0.125 µg of EOMES Recombinant Mouse Monoclonal Antibody (WD1928), Alexa Fluor ™ Plus 488 (Product # 740127MP488). Higher expression of EOMES was observed in NK-92 (pink histogram) in comparison to THP-1 (blue histogram). Viable cells were used for analysis as determined by LIVE/DEAD Fixable Violet Dead Cell Stain Kit (Product # L34964). Flow cytome... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
740127MP488
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1, kappa
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
WD1928
偶联物
Alexa Fluor™ Plus 488
Alexa Fluor™ Plus 488
Alexa Fluor™ Plus 488
激发/发射光谱
493/518 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
规格
50 µg
纯化类型
Protein A/G
保存液
proprietary buffer, pH 6.3-7.3
内含物
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Wet ice
产品详细信息
Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 494 nm; Emission: 519 nm; Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
TBR2 is a member of a conserved protein family that shares a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. A similiar protein disrupted in mice is shown to be essential during trophoblast development and gastrulation. TBR2 plays a role in brain development and is required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: T-box brain protein 2; unnamed protein product
基因别名: TBR2
UniProt ID: (Human) O95936
Entrez Gene ID: (Human) 8320
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
transcription corepressor activity
protein binding
chromatin DNA binding
sequence-specific DNA binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
sequence-specific double-stranded DNA binding
negative regulation of transcription from RNA polymerase II promoter
endoderm formation
mesoderm formation
endodermal cell fate specification
neuron migration
CD8-positive, alpha-beta T cell differentiation involved in immune response
chromatin remodeling
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
brain development
gene expression
cerebral cortex neuron differentiation
cerebral cortex development
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
astrocyte differentiation
cell differentiation involved in embryonic placenta development
mesodermal to mesenchymal transition involved in gastrulation
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