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CD11b Antibody (740175M) in WB
Western blot was performed using CD11b Recombinant Mouse Monoclonal Antibody (Product # 740175M) and a ~165 kDa band corresponding to CD11b was observed upon treatment of U-937 and THP1 with PMA. Membrane-enriched extracts (30 µg lysate) of U-937 (Lane 1), U-937 treated with PMA (10 ng/mL for 3 days) (Lane 2), THP-1 (Lane 3), THP-1 treated with PMA (10 ng/mL for 6 days)(Lane 4), HL-60 (Lane 5), Jurkat (Lane 6) and HeLa (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
740175M
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1, kappa
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
C67F154
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1.0 mg/mL
规格
100 µg
纯化类型
Protein A/G
保存液
PBS, pH 7.2-7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
产品详细信息
Recombinant mouse monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
靶标信息
CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CD11 antigen-like family member B; CD11b; Cell surface glycoprotein MAC-1 subunit alpha; CR-3 alpha chain; Integrin alpha-M; Leukocyte adhesion receptor MO1; Mac-1 alpha (Mac1A); MGC117044; Neutrophil adherence receptor
基因别名: CD11B; CR3A; ITGAM
Entrez Gene ID: (Human) 3684
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