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Invitrogen

Phospho-CHK2 (Thr68) Recombinant Mouse Monoclonal Antibody (ebchk2), Alexa Fluor™ Plus 555

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Product Details

740176MP555

Applications
Tested Dilution
Publications

Immunocytochemistry (ICC/IF)

1:100
-

Flow Cytometry (Flow)

0.5 µg/test
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2b, kappa

Expression System

ExpiCHO

Class

Recombinant Monoclonal

Type

Antibody

Clone

ebchk2

Conjugate

Alexa Fluor™ Plus 555 Alexa Fluor™ Plus 555 Alexa Fluor™ Plus 555

Excitation/Emission Max

558/572 nm View spectra spectra

Form

Liquid

Concentration

1 mg/mL

Amount

50 µg

Purification

Protein A/G

Storage buffer

proprietary buffer, pH 6.3-7.3

Contains

0.008% Bromonitrodioxane, 0.008% Methylisothiazolone

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Wet ice

Product Specific Information

Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.

Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 553 nm; Emission: 568 nm; Laser: Yellow Laser

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CHEK2 (CHK2) is a serine/threonine kinase and a component of the DNA damage checkpoint pathway. A mutation in CHK2 has been linked to cancer. CHK2 is activated by DNA damage and phosphorylates several modulators of cell cycle control including tumor suppressor proteins. In addition, Chk2 can phosphorylate BRCA1, allowing BRCA1 to restore survival after DNA damage. Chk2 is a putative tumor suppressor protein, with mutations in to the Chk2 gene linked to Li-Fraumeni syndrome, a familiar cancer usually associated with inherited mutations in TP53. Also, mutations in CHK2 are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Cds1; Cds1 homolog; Checkpoint kinase 2; Checkpoint like protein CHK2; checkpoint-like protein CHK2; CHEK2; Chk2 (phospho T68); Chk2 (pThr68); CHK2 checkpoint homolog; CHK2 checkpoint homolog (S. pombe); Chk2 phospho; Chk2 phospho T68; Chk2-del2-12; Chk2-del2-3; Chk2-del4; Chk2-del7; Chk2-del9; Chk2-del9-12; Chk2-insX; Chk2-insZ; Chk2-iso1; Chk2-iso2; Chk2-sub3; contains FHA domain; del9; EC 2.7.11.1; HuCds 1; Hucds1; kinase Chk2; OTTHUMP00000199044; OTTHUMP00000199045; OTTHUMP00000199064; OTTHUMP00000199115; OTTHUMP00000199116; phospho Chk2 (T68); phospho Thr68 Chk2; RP11-436C9.1; Serine/threonine protein kinase Chk2; Serine/threonine-protein kinase Chk2; unnamed protein product

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Gene Aliases: CDS1; CHEK2; CHK2; hCds1; HuCds1; LFS2; PP1425; RAD53; TPDS4

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UniProt ID: (Human) O96017

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Entrez Gene ID: (Human) 11200

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Function(s)
nucleotide binding protein kinase activity protein serine/threonine kinase activity protein binding ATP binding kinase activity transferase activity protein kinase binding ubiquitin protein ligase binding identical protein binding protein homodimerization activity metal ion binding protein serine kinase activity
Process(es)
autophagosome assembly DNA damage checkpoint G2/M transition of mitotic cell cycle DNA repair double-strand break repair regulation of transcription, DNA-templated protein phosphorylation apoptotic process cellular response to DNA damage stimulus response to oxidative stress intrinsic apoptotic signaling pathway in response to DNA damage response to gamma radiation protein catabolic process DNA damage response, signal transduction by p53 class mediator intra-S DNA damage checkpoint cellular response to stress regulation of protein catabolic process response to starvation signal transduction in response to DNA damage intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator mitotic DNA damage checkpoint positive regulation of transcription, DNA-templated protein autophosphorylation protein stabilization cell division thymocyte apoptotic process protein K63-linked ubiquitination cellular response to xenobiotic stimulus cellular response to gamma radiation mitotic spindle assembly replicative senescence regulation of signal transduction by p53 class mediator response to glycoside cellular response to bisphenol A negative regulation of DNA damage checkpoint positive regulation of anoikis regulation of autophagosome assembly positive regulation of autophagosome assembly
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