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Invitrogen
FITC Recombinant Mouse Monoclonal Antibody (NAWESLEE)
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FIGURE: 1 / 8
FITC Antibody (740178M) in ICC/IF
Immunofluorescence analysis of FITC was performed using FITC Recombinant Mouse Monoclonal Antibody (NAWESLEE) (Product # 740178M) on HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 min, permeabilized with 0.5% Triton™ X-100 for 15 min and blocked with 2% BSA for 1 hr at RT. The cells were stained with Anti-Ki-67 FITC (Product # 11-5698-82) in 0.1% BSA, incubated at 4ºC overnight. Then the cells were stained with FITC Recombinant Mouse Monoclonal Antibody (NAWESLEE) (Product # 740178M, 1:500, Panel a) at 4ºC overnight, foll... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
740178M
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Host/Isotype
Mouse
/ IgG2a, kappa
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
NAWESLEE
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A/G
Storage buffer
PBS, pH 7.2-7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
Product Specific Information
Recombinant mouse monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Target Information
FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins. Anti-FITC antibodies can be used for isolating FITC labeled cells by magnetic microbeads based sorting, as well as removal of background signal.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Fluorescein
Disclaimer
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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