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Invitrogen

EGR1 Recombinant Rat Monoclonal Antibody (HEGR1DS), Alexa Fluor™ Plus 647

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Product Details

740183TP647

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500
-

Immunocytochemistry (ICC/IF)

1:200
-

Flow Cytometry (Flow)

1 µg/test
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rat / IgG2a, kappa

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

HEGR1DS

Conjugate

Alexa Fluor™ Plus 647 Alexa Fluor™ Plus 647 Alexa Fluor™ Plus 647

Excitation/Emission Max

658/675 nm View spectra spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

proprietary buffer, pH 6.3-7.3

Contains

0.008% Bromonitrodioxane, 0.008% Methylisothiazolone

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Wet ice

Product Specific Information

Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.

Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 658 nm; Emission: 675 nm; Laser: Red Laser

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppresor gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: AT225; Early growth response protein 1; EGR-1; ETR103; Nerve growth factor-induced protein A; NGFI-A; Transcription factor ETR103; Transcription factor Zif268; unnamed protein product; zinc finger gene 225; Zinc finger protein 225; Zinc finger protein Krox-24

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Gene Aliases: AT225; EGR1; G0S30; KROX-24; KROX24; NGFI-A; TIS8; ZIF-268; ZIF268; ZNF225

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UniProt ID: (Human) P18146

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Entrez Gene ID: (Human) 1958

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Function(s)
transcription regulatory region sequence-specific DNA binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding zinc ion binding double-stranded methylated DNA binding enzyme binding histone acetyltransferase binding sequence-specific DNA binding hemi-methylated DNA-binding metal ion binding sequence-specific double-stranded DNA binding promoter-specific chromatin binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter response to hypoxia response to ischemia regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter response to glucose positive regulation of gene expression T cell differentiation BMP signaling pathway positive regulation of chemokine production positive regulation of interleukin-1 beta production response to insulin circadian regulation of gene expression regulation of protein sumoylation skeletal muscle cell differentiation regulation of apoptotic process regulation of neuron apoptotic process hypomethylation of CpG island estrous cycle locomotor rhythm positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of hormone biosynthetic process rhythmic process circadian temperature homeostasis interleukin-1-mediated signaling pathway cellular response to gamma radiation cellular response to heparin cellular response to mycophenolic acid glomerular mesangial cell proliferation positive regulation of glomerular metanephric mesangial cell proliferation negative regulation of canonical Wnt signaling pathway cellular response to interleukin-8 positive regulation of post-translational protein modification positive regulation of pri-miRNA transcription from RNA polymerase II promoter regulation of progesterone biosynthetic process
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