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Invitrogen
N-Cadherin Recombinant Mouse Monoclonal Antibody (3B9)
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FIGURE: 1 / 4
N-Cadherin Antibody (750016M) in WB
Western blot was performed using N-cadherin Recombinant Mouse Monoclonal Antibody (3B9) (Product # 750016M) and a ~130 kDa band corresponding to N-cadherin was observed in Hep G2, SK-N-SH and SH-SY5Y cell lines as compared to HeLa, Caco-2 and Raji cell line which are reported to be negative for N-cadherin expression. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), Hep G2 (Lane 2), SK-N-SH (Lane 3), Caco-2 (Lane 4), SH-SY5Y (Lane 5), Raji (Lane 6), tissue extracts (30 µg lysate) of mouse heart (Lane 7) and mouse brain (Lane 8) were ele... View More
Product Details
750016M
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
3B9
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A/G
Storage buffer
PBS, pH 7.2-7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
Product Specific Information
Recombinant mouse monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Target Information
N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. N-cadherin's function is dependent on its association with the actin-cytoskeleton and is mediated through interactions between the C-terminal region of N-cadherin and the cytoplasmic catenin proteins. The stability of this association is regulated by phosphorylation and dephosphorylation of beta-catenin. Further, N-cadherin is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cadherin 2, type 1, N-cadherin (neuronal); Cadherin-2; Cadherin-N; Cadherin2; calcium-dependent adhesion protein, neuronal; CD325; CDw325; CDw325 antigen; N-cadherin; N-cadherin 1; Neural cadherin; unnamed protein product
Gene Aliases: ACOGS; ADHD8; ARVD14; CD325; CDH2; CDHN; CDw325; NCAD
UniProt ID: (Human) P19022
Entrez Gene ID: (Human) 1000
cell morphogenesis
type B pancreatic cell development
cell-cell junction assembly
cell adhesion
homophilic cell adhesion via plasma membrane adhesion molecules
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
synapse assembly
glial cell differentiation
neural crest cell development
calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules
cell migration
telencephalon development
cerebral cortex development
adherens junction organization
detection of muscle stretch
positive regulation of MAPK cascade
cell-cell adhesion mediated by cadherin
blood vessel morphogenesis
brain morphogenesis
homeostasis of number of cells
striated muscle cell differentiation
radial glial cell differentiation
neuroepithelial cell differentiation
regulation of oligodendrocyte progenitor proliferation
protein localization to plasma membrane
negative regulation of canonical Wnt signaling pathway
mesenchymal cell migration
synaptic vesicle clustering
neuroligin clustering involved in postsynaptic membrane assembly
neuronal stem cell population maintenance
cell-cell adhesion
regulation of postsynaptic density protein 95 clustering
positive regulation of synaptic vesicle clustering
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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