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Invitrogen
HuC/HuD Monoclonal Antibody (16A11), Biotin
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HuC/HuD Antibody (A-21272) in IHC
Mouse brain sections were labeled with the NeuroTrace® 500/525 green-fluorescent Nissl stain (Product # N-21480) or with our biotin-XX conjugate of anti-HuC/HuD antibody, followed by visualization with Alexa Fluor® 568 streptavidin (Product # S-11226). Both sections were counterstained with DAPI (Product # D1306, D3571, D21490) (Product # A-21272)
产品信息
A-21272
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
C. elegans,
Fish,
Guinea pig,
Human,
Mouse,
Pig,
Rat,
Zebrafish
宿主/亚型
Mouse
/ IgG2b, kappa
分类
Monoclonal
类型
Antibody
克隆号
16A11
抗原
Human HuC/HuD neuronal protein.
偶联物
形式
Lyophilized
纯化类型
purified
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
This antibody recognizes the Elav family members HuC, HuD and Hel-N1 neuronal proteins. It does not recognize HuR, another Elav family member that is present in all proliferating cells. The antibody has been shown to specifically label neuronal cells in zebrafish, chick, canaries, and humans, and is likely to label neuronal cells in most vertebrate species. Labeling is visible early in development, at about the time that the neurons leave the mitotic cycle.
Storage and reconstitution: Upon receipt, lyophilized antibodies should be stored desiccated at -20°C or lower. When properly stored, these products are stable for at least one year. To prepare stock solution, reconstitute the antibody in 0.5-1 mL PBS, pH 7.4, containing 1% BSA. Store the solution for up to two weeks at 4°C with the addition of 2mM sodium azide. For longer storage, divide solutions into single-use aliquots and freeze at -20°C, avoidig freeze/thaw cycles.
For best results in immunohistochemistry, antigen retrieval is required. Use 50mM Tris, pH 8.0, for 30 minutes. The biotin-XX labeled antibody can be detected by using fluorescently labeled avidin, streptavidin or NeutrAvidin conjugates.
靶标信息
Anti-Hu antibodies were originally isolated from a patient with paraneoplastic encephalomyelitis, a rare condition of selective neural tissue injury associated with small cell lung carcinoma. The antibodies bind specifically to antigens present exclusively in neuronal cells and are thus useful as markers of neuronal cells in tissue. The Hu antigen is an RNA-binding protein of the embryonic lethal abnormal visual (Elav) family.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: ELAV (embryonic lethal, abnormal vision, Drosophila)-like 3 (Hu antigen C); ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D); ELAV-like protein 3; ELAV-like protein 4; Hu antigen C; Hu antigen D; Hu-antigen C; Hu-antigen D; HuC; HuD; Paraneoplastic cerebellar degeneration-associated antigen; Paraneoplastic encephalomyelitis antigen HuD; Paraneoplastic limbic encephalitis antigen 21
基因别名: ELAVL3; ELAVL4; HUC; HUCL; HUD; PLE21; PNEM
UniProt ID: (Human) Q14576, (Human) P26378
Entrez Gene ID: (Human) 1995, (Human) 1996
