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GenScript
DYKDDDDK Tag Monoclonal Antibody (5A8E5), FITC, THE™
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产品信息
A01632
应用
建议稀释比
已发表文章
产品规格
种属反应
Tag
宿主/亚型
Mouse
/ IgG2b, kappa
分类
Monoclonal
类型
Antibody
克隆号
5A8E5
抗原
A synthetic peptide DYKDDDDK coupled to KLH
偶联物
激发/发射光谱
498/517 nm
查看光谱
形式
Lyophilized
纯化类型
Protein A
保存液
PBS, pH 7.4, with 1% BSA
内含物
0.02% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles, store in dark
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/mL.
GenScript THE™ DYKDDDDK Tag Antibody [FITC], mAb, Mouse is FITC conjugated THE™ DYKDDDDK Tag Antibody, mAb, Mouse (A000187) purified from mice ascites by Protein A affinity column. This product is suitable for detecting the expression level of DYKDDDDK fusion proteins by various immunoassays. Absorption Wavelength: 492 nmEmission Wavelength: 518 nmFluorochrome/Protein Ratio: >3 moles FITC per mole antibody
Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below.
靶标信息
DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization. The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. It is often fused to the N- or C-terminus of a protein of interest, allowing for easy identification and isolation of the protein using anti-FLAG antibodies. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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