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Invitrogen
PDGFRA Monoclonal Antibody (16A1), PE
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FIGURE: 1 / 2
PDGFRA Antibody (A15785) in Flow
Flow cytometry analysis of PDGFRA in 3T3 cells. Samples were incubated with PDGFRA PE monoclonal antibody (Product # A15785) using a dilution of 6 μg/mL (red-filled histogram). Antibody binds specifically to CD140a expressed on surface of PDGFRA transfected 3T3 cells (upper panel), but not to surface of non-transfected 3T3 cells (lower panel). Level of non-specific binding was assessed using Mouse IgG1 isotype control PE antibody under same conditions (concentration in sample 6 μg/mL, black-dashed histogram).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
A15785
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
16A1
Immunogen
CD140a-transfected NIH 3T3 cells
Conjugate
PE
PE
PE
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Purification
Size-exclusion chromatography
Storage buffer
PBS, pH 7.4, with 0.2% BSA
Contains
15mM sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody recognizes an extracellular epitope of CD140a / PDGF-RA, the 170 kDa alpha chain of platelet-derived growth factor receptor, which is widely expressed on a variety of mesenchymal-derived cells and plays pro-proliferative or anti-proliferative roles in various tumors.
Target Information
PDGFRA is a cell surface receptor tyrosine kinase which binds members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MGC74795; OTTHUMP00000218656; PDGF A-chain; PDGF Receptor alpha; PDGF subunit A; PDGF-1; pdgfr-a
Disclaimer
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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