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Rockland
AAK1 Polyclonal Antibody
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FIGURE: 1 / 1
AAK1 Antibody (600-401-X53) in WB
Western Blot of Aak1 antibody. Lane A: A20 cell lysate at 1 µg/mL. Lane B: A20 cell lysate at 2 µg/mL. Load: 35 µg per lane. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 103.8 kDa, ~86 kDa for Aak1.
Product Details
600-401-X53
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-Aak1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 18 amino acid synthetic peptide near the C-terminus of the human Aak1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
AAK1 serine/threonine kinase is a member of the NAK family and plays a regulatory role in endocytosis. AAK1 is a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Adaptor-associated kinase 1; AP2 associated kinase 1; AP2-associated protein kinase 1; DKFZp686F03202; DKFZp686K16132; FLJ23712; FLJ25931; FLJ31060; FLJ42882; FLJ45252; MGC138170; MGC164568; MGC164570
Gene Aliases: 5530400K14Rik; 9630042K20; AAK1; AU067724; AU067726; BC028270; C79663; D6Ertd245e; KIAA1048; mKIAA1048; R75501; RGD1563580
UniProt ID: (Human) Q2M2I8, (Mouse) Q3UHJ0
Entrez Gene ID: (Human) 22848, (Mouse) 269774, (Rat) 500244
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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