Invitrogen

ABHD6 Recombinant Rabbit Monoclonal Antibody (1U1I2)

查看其他4个ABHD6抗体

问题与答案

问题与答案

问题与答案

Additional Information:

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抗体检测数据 (6)

ABHD6 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ABHD6 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

图: 1 / 6

ABHD6 Antibody (MA569744) in IHC (P)

Immunohistochemistry analysis of ABHD6 on paraffin-embedded Rat liver tissue. Samples were incubated with ABHD6 recombinant monoclonal antibody (Product # MA569744) using a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0. 01 M Tris-EDTA Buffer (pH 9. 0).

ABHD6 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ABHD6 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ABHD6 Antibody in Immunohistochemistry (Frozen) (IHC (F))

ABHD6 Antibody in Western Blot (WB)

ABHD6 Antibody in Western Blot (WB)

ABHD6 Antibody in Western Blot (WB)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

ABHD6 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of ABHD6 on paraffin-embedded Rat liver tissue. Samples were incubated with ABHD6 recombinant monoclonal antibody (Product # MA569744) using a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0. 01 M Tris-EDTA Buffer (pH 9. 0).

ABHD6 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of ABHD6 on paraffin-embedded Rat brain tissue. Samples were incubated with ABHD6 recombinant monoclonal antibody (Product # MA569744) using a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0. 01 M Tris-EDTA Buffer (pH 9. 0).

ABHD6 Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Immunohistochemistry analysis of ABHD6 on frozen sections Mouse brain tissue. Samples were incubated with ABHD6 recombinant monoclonal antibody (Product # MA569744) using a dilution of 1:200 (40x lens). Followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) using a dilution of 1:500 (Red). DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0).

ABHD6 Antibody in Western Blot (WB) — Western blot analysis of ABHD6 in Mouse liver and Mouse brain cells. Samples were probed with ABHD6 recombinant monoclonal antibody (Product # MA569744) at 1:6,000 dilution incubated overnight at 4°C. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

ABHD6 Antibody in Western Blot (WB) — Western blot analysis of ABHD6 in wild type (WT) and Abhd6 Mouse brain. Samples were probed with ABHD6 recombinant monoclonal antibody (Product # MA569744) at 1:6,000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

ABHD6 Antibody in Western Blot (WB) — Western blot analysis of ABHD6 in RPMI 8226 cells. Samples were probed with ABHD6 recombinant monoclonal antibody (Product # MA569744) at 1:6,000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

产品信息

MA569744

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:6,000-1:24,000

-

免疫组化（石蜡） (IHC (P))

1:100-1:400

-

免疫组化（冰冻） (IHC (F))

1:100-1:400

-

酶联免疫吸附实验 (ELISA)

1 µg/mL

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

1U1I2

抗原

Recombinant protein (or fragment).

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.2 mg/mL

120 µg

纯化类型

Affinity chromatography

保存液

PBS, pH 7.3., with 50% glycerol

内含物

0.09% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

靶标信息

ABHD6 has 2-arachidonoylglycerol hydrolase activity. ABHD6 may be a regulator of endocannabinoid signaling pathways.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: 2-arachidonoylglycerol hydrolase; abhydrolase domain-containing protein 6; lipase protein; unnamed protein product

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基因别名: 0610041D24Rik

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UniProt ID: (Human) Q9BV23, (Mouse) Q8R2Y0, (Rat) Q5XI64

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Entrez Gene ID: (Human) 57406, (Mouse) 66082, (Rat) 305795

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catalytic activity phospholipase activity protein binding acylglycerol lipase activity

phospholipid catabolic process negative regulation of cell migration acylglycerol catabolic process positive regulation of lipid biosynthetic process monoacylglycerol catabolic process long term synaptic depression regulation of retrograde trans-synaptic signaling by endocanabinoid negative regulation of cold-induced thermogenesis regulation of endocannabinoid signaling pathway lysobisphosphatidic acid metabolic process arachidonic acid metabolic process