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Agrisera
ABI5/Abscisic acid insensitive 5 Polyclonal Antibody
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ABI5/Abscisic acid insensitive 5 Antibody (AS12-1863) in WB
2,5 and 10 µg of total protein (run on separate lanes) extracted from the Columbia ecotype (Arabidopsis thaliana) seeds using Acetone extraction was separated using the Bolt® Bis-Tris Plus Gel system on a 4-12% gradient SDS-PAGE gel, blotted using the turbo-blot system (BIO-RAD) to transfer onto a PVDF membrane (7 min). SNAP-ID (Millipore) system was used for blocking and antibody labeling. Blocking occurre... View More
产品信息
AS12-1863
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide derived from Arabidopsis thaliana ABI5 sequence.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
规格
100 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
ABI5 is a protein-coding gene in Arabidopsis thaliana whose product localizes predominantly to the nucleus and functions as a basic leucine zipper (bZIP) transcription factor in abscisic acid (ABA) signaling during seed maturation, germination, and early post-germinative growth. The locus produces multiple transcripts, and the canonical protein contains a C-terminal plant bZIP DNA-binding and dimerization module (basic region plus leucine zipper) that is required for sequence-specific binding to ABA-responsive promoter elements and for transcription factor dimer formation. Genetic and molecular studies show that loss-of-function abi5 alleles reduce sensitivity to ABA-mediated inhibition of germination and perturb expression of subsets of ABA-regulated genes, including late embryogenesis-abundant (LEA) stress-adaptation genes, consistent with ABI5 acting downstream of the core ABA pathway and integrating developmental and environmental cues to enforce a post-germination growth arrest/checkpoint under unfavorable conditions.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ABA INSENSITIVE 5; AtABI5; F2H17.12; F2H17_12; GIA1; GROWTH-INSENSITIVITY TO ABA 1
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