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ACADM Antibody (PA5-119675) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of of ACADM in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with ACADM Polyclonal Antibody (Product # PA5-119675) at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue).
产品信息
PA5-119675
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant protein within Human ACADM aa 13-239
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol, 0.2% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2913248
产品详细信息
Positive Control: Daudi cell lysate, K562 cell lysate, SH-SY5Y, human liver carcinoma tissue, mouse kidney tissue, Hela.
Subcellular Location: Mitochondrion matrix.
靶标信息
This gene encodes the medium-chain specific (C4 to C12 straight chain) acyl-Coenzyme A dehydrogenase. The homotetramer enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Defects in this gene cause medium-chain acyl-CoA dehydrogenase deficiency, a disease characterized by hepatic dysfunction, fasting hypoglycemia, and encephalopathy, which can result in infantile death. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: acyl-CoA dehydrogenase C-4 to C-12 straight chain; acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain; FLJ18227; FLJ93013; FLJ99884; MCAD; MCADH; Medium chain acyl-CoA dehydrogenase; Medium-chain specific acyl-CoA dehydrogenase, mitochondrial; RP4-682C21.1; testicular tissue protein Li 7
基因别名: ACAD1; ACADM; AU018656; MCAD; MCADH
UniProt ID: (Human) P11310, (Mouse) P45952
Entrez Gene ID: (Human) 34, (Mouse) 11364
fatty-acyl-CoA binding
acyl-CoA dehydrogenase activity
electron carrier activity
identical protein binding
flavin adenine dinucleotide binding
oxidoreductase activity, acting on the CH-CH group of donors, with a flavin as acceptor
medium-chain-acyl-CoA dehydrogenase activity
oxidoreductase activity
oxidoreductase activity, acting on the CH-CH group of donors
isomerase activity
dehydrogenase
fatty acid beta-oxidation
carnitine metabolic process, CoA-linked
fatty acid beta-oxidation using acyl-CoA dehydrogenase
carnitine biosynthetic process
medium-chain fatty acid metabolic process
medium-chain fatty acid catabolic process
lipid homeostasis
oxidation-reduction process
liver development
glycogen biosynthetic process
organic acid metabolic process
regulation of gluconeogenesis
lipid metabolic process
fatty acid metabolic process
heart development
metabolic process
response to cold
carnitine metabolic process
post-embryonic development
response to starvation
protein homotetramerization
cardiac muscle cell differentiation
