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Invitrogen
ACSL3 Polyclonal Antibody
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FIGURE: 1 / 2
ACSL3 Antibody (PA5-114815) in ICC/IF
Immunocytochemistry analysis of ACSL3 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114815). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conjugated goat anti-mouse IgG (Green) and DAPI.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-114815
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ACSL3(Accession O95573), corresponding to amino acid residues N493-E543.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total ACSL3.
Target Information
An initial reaction in fatty acid metabolism in eukaryotic cells is activation of fatty acids catalyzed by acyl-CoA synthetase. FACL3 (fatty acid CoA ligase, long-chain 3) is identified as member of the acyl-CoA synthetase (ACS) family by PCR of rat brain cDNAs using primers based on the conserved region of the ACS protein. The 720-amino acid rat protein preferentially utilizes myristate, laurate, arachidonate, and eicosapentaenoate, and is expressed primarily in brain. The predicted 720-amino acid FACL3 human protein is 92% identical to that of rat.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Arachidonate--CoA ligase; Arachidonate--CoA ligase Acsl3; Brain acyl-CoA synthetase II; Fatty acid CoA ligase Acsl3; fatty acid Coenzyme A ligase, long chain 3; LACS 3; Long-chain acyl-CoA synthetase 3; Long-chain-fatty-acid--CoA ligase 3; Medium-chain acyl-CoA ligase Acsl3
Gene Aliases: 2610510B12Rik; ACS3; ACSL3; C85929; FACL3; LACS3; Pro2194
UniProt ID: (Rat) Q63151, (Mouse) Q9CZW4
Entrez Gene ID: (Rat) 114024, (Mouse) 74205
long-chain fatty acid metabolic process
lipid metabolic process
fatty acid metabolic process
fatty acid biosynthetic process
brain development
response to nutrient
metabolic process
response to organic cyclic compound
very-low-density lipoprotein particle assembly
positive regulation of Golgi to plasma membrane protein transport
long-chain fatty acid import
positive regulation of secretion
positive regulation of phosphatidylcholine biosynthetic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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